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HRD1/SYVN1 Monoclonal antibody, PBS Only

HRD1/SYVN1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C8A7

Cat No : 67488-1-PBS

Print datasheet

Synonyms

HRD1, HRD1/SYVN1, KIAA1810, Synovial apoptosis inhibitor 1, SYVN1

验证数据展示

  • WB analysis using 67488-1-Ig (same clone as 67488-1-PBS)

    WB analysis using 67488-1-Ig (same clone as 67488-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67488-1-Ig (same clone as 67488-1-PBS)

    IHC staining of human liver cancer using 67488-1-Ig (same clone as 67488-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67488-1-Ig (same clone as 67488-1-PBS)

    IHC staining of human liver cancer using 67488-1-Ig (same clone as 67488-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67488-1-Ig (same clone as 67488-1-PBS)

    IF Staining of HeLa using 67488-1-Ig (same clone as 67488-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HRD1/SYVN1 antibody (67488-1-Ig, Clone: 2C8A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

产品信息

67488-1-PBS targets HRD1/SYVN1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen HRD1/SYVN1 fusion protein Ag4456 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name synovial apoptosis inhibitor 1, synoviolin
Synonyms HRD1, HRD1/SYVN1, KIAA1810, Synovial apoptosis inhibitor 1, SYVN1
Calculated Molecular Weight 617 aa, 68 kDa
Observed Molecular Weight 68-76 kDa
GenBank Accession NumberBC030530
Gene Symbol SYVN1
Gene ID (NCBI) 84447
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86TM6
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).