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验证数据展示

  • WB analysis using 67488-1-Ig (same clone as 67488-1-PBS)

    WB analysis using 67488-1-Ig (same clone as 67488-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67488-1-Ig (same clone as 67488-1-PBS)

    IHC staining of human liver cancer using 67488-1-Ig (same clone as 67488-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYVN1/HRD1 antibody included
  • IHC staining of human liver cancer using 67488-1-Ig (same clone as 67488-1-PBS)

    IHC staining of human liver cancer using 67488-1-Ig (same clone as 67488-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYVN1/HRD1 antibody included
  • WB analysis of HeLa using 67488-1-Ig (same clone as 67488-1-PBS)

    WB analysis of HeLa using 67488-1-Ig (same clone as 67488-1-PBS)

    WB result of HRD1/SYVN1 antibody (67488-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRD1/SYVN1 transfected HeLa cells. This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67488-1-Ig (same clone as 67488-1-PBS)

    IF Staining of HeLa using 67488-1-Ig (same clone as 67488-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HRD1/SYVN1 antibody (67488-1-Ig, Clone: 2C8A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

HRD1/SYVN1 Monoclonal antibody, PBS Only

HRD1/SYVN1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67488-1-PBS

产品说明书

CloneNo. 2C8A7

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

SYVN1, Synovial apoptosis inhibitor 1, KIAA1810, HRD1, EC:2.3.2.27

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SYVN1/HRD1 Ready-To-Use IHC Kit. SYVN1/HRD1 primary antibody included.

产品信息

67488-1-PBS targets HRD1/SYVN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HRD1/SYVN1 fusion protein Ag4456 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 synovial apoptosis inhibitor 1, synoviolin
别名 SYVN1, Synovial apoptosis inhibitor 1, KIAA1810, HRD1, EC:2.3.2.27
计算分子量 617 aa, 68 kDa
观测分子量 68-76 kDa
GenBank蛋白编号BC030530
基因名称 HRD1
Gene ID (NCBI) 84447
RRIDAB_2882714
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86TM6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).