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HRG Polyclonal antibody

HRG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26252-1-AP

Print datasheet

Synonyms

histidine rich glycoprotein, HPRG, HRG, HRGP

验证数据展示

  • WB analysis of human plasma using 26252-1-AP

    WB analysis of human plasma using 26252-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 26252-1-AP (HRG antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 26252-1-AP

    IHC staining of mouse liver using 26252-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26252-1-AP (HRG antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

26252-1-AP targets HRG in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Immunogen HRG fusion protein Ag16278 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histidine-rich glycoprotein
Synonyms histidine rich glycoprotein, HPRG, HRG, HRGP
Calculated Molecular Weight 525 aa, 60 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC069574
Gene Symbol HRG
Gene ID (NCBI) 3273
RRIDAB_2880447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04196
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HRG antibody 26252-1-APDownload protocol
IHC protocol for HRG antibody 26252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO Mol Med

Histidine-rich glycoprotein modulates neutrophils and thrombolysis-associated hemorrhagic transformation

Authors - Wei Jiang
View Article
rat

Front Pharmacol

17β-Estradiol Enhances Schwann Cell Differentiation via the ERβ-ERK1/2 Signaling Pathway and Promotes Remyelination in Injured Sciatic Nerves.

Authors - Yun Gu
View Article