验证数据展示
经过测试的应用
| Positive WB detected in | human colon tissue, Jurkat cells |
| Positive IHC detected in | human endometrial cancer tissue, human colon cancer tissue, human trachea tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13413-1-AP targets HRH1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | HRH1 fusion protein Ag4067 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | histamine receptor H1 |
| 别名 | H1 R, H1R, HH1R, hisH1, Histamine H1 receptor, histamine receptor H1, HRH1 |
| 计算分子量 | 487 aa, 56 kDa |
| 观测分子量 | 56 kDa |
| GenBank蛋白编号 | BC060802 |
| 基因名称 | HRH1 |
| Gene ID (NCBI) | 3269 |
| RRID | AB_2092454 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35367 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HRH1 antibody 13413-1-AP | Download protocol |
| IHC protocol for HRH1 antibody 13413-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Stem Cell Reports Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids. | ||
Aging (Albany NY) Combined impacts of histamine receptor H1 gene polymorphisms and an environmental carcinogen on the susceptibility to and progression of oral squamous cell carcinoma. | ||
Front Cell Neurosci Clemastine Alleviates Depressive-Like Behavior Through Reversing the Imbalance of Microglia-Related Pro-inflammatory State in Mouse Hippocampus. | ||
J Virol Kaposi's Sarcoma-Associated Herpesvirus Infection Induces the Expression of Neuroendocrine Genes in Endothelial Cells.
| ||
Am J Physiol Cell Physiol Functional role of histamine receptors in the renal cortical collecting duct cells. |
