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  • Featured Product
  • KD/KO Validated

HRPT2, CDC73 Monoclonal antibody, PBS Only

HRPT2, CDC73 Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Mouse, Rat

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

4B4H2

Cat No : 66490-1-PBS

Print datasheet

Synonyms

C1orf28, CDC73, HPT JT, HRPT2, HRPT2; CDC73, Hyperparathyroidism 2 protein, Parafibromin

验证数据展示

  • WB analysis of HeLa using 66490-1-Ig (same clone as 66490-1-PBS)

    WB analysis of HeLa using 66490-1-Ig (same clone as 66490-1-PBS)

    WB result of HRPT2, CDC73 antibody (66490-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRPT2, CDC73 transfected HeLa cells. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

    IHC staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDC73 antibody included
  • IHC staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

    IHC staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66490-1-Ig (HRPT2; CDC73 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDC73 antibody included
  • IF Staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

    IF Staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using HRPT2, CDC73 antibody (66490-1-Ig, Clone: 4B4H2 ) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

  • WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

    WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

  • WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

    WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

  • WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

    WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CDC73 Ready-To-Use IHC Kit. CDC73 primary antibody included.

产品信息

66490-1-PBS targets HRPT2, CDC73 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Applications WB,IHC,IF,ELISA Application Description
Tested Reactivity Human, Pig, Mouse, Rat
Immunogen HRPT2, CDC73 fusion protein Ag2959 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Synonyms C1orf28, CDC73, HPT JT, HRPT2, HRPT2; CDC73, Hyperparathyroidism 2 protein, Parafibromin
Calculated Molecular Weight 531 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC014351
Gene Symbol CDC73
Gene ID (NCBI) 79577
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6P1J9
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

Parafibromin is a product of the hyperparathyroidism-jaw tumor syndrome gene HRPT2/CDC73, a putative tumor suppressor gene recently implicated in the autosomal dominant hyperparathyroidism-jaw tumor familial cancer syndrome, sporadic parathyroid cancer, and a minority of families with isolated hyperparathyroidism (PMID: 15580289). Defects in CDC73 are causes of hyperparathyroidism type 1 (HRPT1) and hyperparathyroidism type 2 (HRPT2) as well as parathyroid carcinoma (PRTC) (PMID: 12434154). Tumor suppressor parafibromin to the transcription elongation and RNA processing pathway as a PAF1 complex- and RNA polymerase II-bound protein (PMID: 15923622). Besides, parafibromin is also involved in post-transcriptional control pathways (PMID: 16116486). Recent report has revealed that pathogenic mutation, such as CDC73 gene mutation, is able to affect histone monoubiquitination (PMID: 22021426).