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  • Featured Product
  • KD/KO Validated

HRPT2, CDC73 Monoclonal antibody, PBS Only

HRPT2, CDC73 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Mouse, Rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

4B4H2

Cat no : 66490-1-PBS

Print datasheet

Synonyms

C1orf28, CDC73, HPT JT, HRPT2, HRPT2; CDC73, Hyperparathyroidism 2 protein, Parafibromin

验证数据展示

  • WB analysis of HeLa using 66490-1-Ig (same clone as 66490-1-PBS)

    WB analysis of HeLa using 66490-1-Ig (same clone as 66490-1-PBS)

    WB result of HRPT2, CDC73 antibody (66490-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRPT2, CDC73 transfected HeLa cells. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

    IHC staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDC73 antibody included
  • IHC staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

    IHC staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66490-1-Ig (HRPT2; CDC73 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDC73 antibody included
  • IF Staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

    IF Staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using HRPT2, CDC73 antibody (66490-1-Ig, Clone: 4B4H2 ) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

  • WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

    WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

  • WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

    WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

  • WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

    WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CDC73 Ready-To-Use IHC Kit. CDC73 primary antibody included.

产品信息

66490-1-PBS targets HRPT2, CDC73 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity Human, Pig, Mouse, Rat
Immunogen HRPT2, CDC73 fusion protein Ag2959 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Synonyms C1orf28, CDC73, HPT JT, HRPT2, HRPT2; CDC73, Hyperparathyroidism 2 protein, Parafibromin
Calculated Molecular Weight 531 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC014351
Gene Symbol HRPT2/CDC73
Gene ID (NCBI) 79577
RRIDAB_2881855
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6P1J9
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Parafibromin is a product of the hyperparathyroidism-jaw tumor syndrome gene HRPT2/CDC73, a putative tumor suppressor gene recently implicated in the autosomal dominant hyperparathyroidism-jaw tumor familial cancer syndrome, sporadic parathyroid cancer, and a minority of families with isolated hyperparathyroidism (PMID: 15580289). Defects in CDC73 are causes of hyperparathyroidism type 1 (HRPT1) and hyperparathyroidism type 2 (HRPT2) as well as parathyroid carcinoma (PRTC) (PMID: 12434154). Tumor suppressor parafibromin to the transcription elongation and RNA processing pathway as a PAF1 complex- and RNA polymerase II-bound protein (PMID: 15923622). Besides, parafibromin is also involved in post-transcriptional control pathways (PMID: 16116486). Recent report has revealed that pathogenic mutation, such as CDC73 gene mutation, is able to affect histone monoubiquitination (PMID: 22021426).