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HS3ST1 Polyclonal antibody

HS3ST1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14358-1-AP

Print datasheet

Synonyms

3 OST 1, 3OST, 3OST1, h3 OST 1, HS3ST1

验证数据展示

  • WB analysis of mouse spleen using 14358-1-AP

    WB analysis of mouse spleen using 14358-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14358-1-AP (HS3ST1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 14358-1-AP

    IHC staining of human heart using 14358-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14358-1-AP (HS3ST1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 14358-1-AP

    IHC staining of human heart using 14358-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14358-1-AP (HS3ST1 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse spleen tissue
Positive IHC detected inhuman heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14358-1-AP targets HS3ST1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen HS3ST1 fusion protein Ag5719 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heparan sulfate (glucosamine) 3-O-sulfotransferase 1
Synonyms 3 OST 1, 3OST, 3OST1, h3 OST 1, HS3ST1
Calculated Molecular Weight 36 kDa
GenBank Accession NumberBC057803
Gene Symbol HS3ST1
Gene ID (NCBI) 9957
RRIDAB_10805763
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14792
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HS3ST1 antibody 14358-1-APDownload protocol
IHC protocol for HS3ST1 antibody 14358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Oncol

Development and validation of a novel hypoxia-related signature for prognostic and immunogenic evaluation in head and neck squamous cell carcinoma

Authors - Su-Ran Li
View Article
humanIF

Sci Rep

Switching mechanism from AR to EGFR signaling via 3-O-sulfated heparan sulfate in castration-resistant prostate cancer

Authors - Hayato Ota
View Article