HSD17B1 Polyclonal antibody
HSD17B1 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | human placenta tissue |
Positive IP detected in | human placenta tissue |
Positive IHC detected in | human placenta tissue, human colon cancer tissue, human endometrial cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 4 publications below |
产品信息
25334-1-AP targets HSD17B1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IP, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Immunogen | HSD17B1 fusion protein Ag17886 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | hydroxysteroid (17-beta) dehydrogenase 1 |
Synonyms | 20 alpha-hydroxysteroid dehydrogenase, 20 alpha HSD, 17β-HSD, 17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1 |
Calculated Molecular Weight | 328 aa, 35 kDa |
Observed Molecular Weight | 35 kDa |
GenBank Accession Number | BC104752 |
Gene Symbol | HSD17B1 |
Gene ID (NCBI) | 3292 |
RRID | AB_2880032 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P14061 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for HSD17B1 antibody 25334-1-AP | Download protocol |
IHC protocol for HSD17B1 antibody 25334-1-AP | Download protocol |
IP protocol for HSD17B1 antibody 25334-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Transl Med An integrated approach of network pharmacology, molecular docking, and experimental verification uncovers kaempferol as the effective modulator of HSD17B1 for treatment of endometrial cancer | ||
Cells HDAC1-Mediated lncRNA Stimulatory Factor of Follicular Development to Inhibit the Apoptosis of Granulosa Cells and Regulate Sexual Maturity through miR-202-3p-COX1 Axis | ||