HSD17B1 Polyclonal antibody

HSD17B1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25334-1-AP

Print datasheet

Synonyms

20 alpha-hydroxysteroid dehydrogenase, 20 alpha HSD, 17β-HSD, 17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1



经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue, human colon cancer tissue, human endometrial cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

25334-1-AP targets HSD17B1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen HSD17B1 fusion protein Ag17886 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hydroxysteroid (17-beta) dehydrogenase 1
Synonyms 20 alpha-hydroxysteroid dehydrogenase, 20 alpha HSD, 17β-HSD, 17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1
Calculated Molecular Weight 328 aa, 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC104752
Gene Symbol HSD17B1
Gene ID (NCBI) 3292
RRIDAB_2880032
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14061
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HSD17B1 antibody 25334-1-APDownload protocol
IHC protocol for HSD17B1 antibody 25334-1-APDownload protocol
IP protocol for HSD17B1 antibody 25334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Transl Med

An integrated approach of network pharmacology, molecular docking, and experimental verification uncovers kaempferol as the effective modulator of HSD17B1 for treatment of endometrial cancer

Authors - Guan-Yu Ruan
mouseWB

Cells

HDAC1-Mediated lncRNA Stimulatory Factor of Follicular Development to Inhibit the Apoptosis of Granulosa Cells and Regulate Sexual Maturity through miR-202-3p-COX1 Axis

Authors - Xiaofeng Zhou
mouseWB

J Nanobiotechnology

Ti3C2 nanosheet-induced autophagy derails ovarian functions

Authors - Limei Yang
mouseWB

Sci Total Environ

Polystyrene microplastics trigger testosterone decline via GPX1

Authors - Jiayuan Qu