验证数据展示

WB analysis of HEK-293 using 10648-1-AP
WB result of ERAB antibody (10648-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERAB transfected HEK-293 cells.
WB analysis of HeLa using 10648-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10648-1-AP (ERAB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 10648-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10648-1-AP (ERAB antibody) at dilution of 1:100 (under 10x lens).
Ready-to-use IHC kits available with HSD17B10 antibody included
IP experiment of HeLa using 10648-1-AP
IP result of anti-ERAB (IP:10648-1-AP, 4ug; Detection:10648-1-AP 1:500) with HeLa cells lysate 1200ug.
IHC staining of mouse brain using 10648-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10648-1-AP (ERAB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with HSD17B10 antibody included
IF Staining of MCF-7 using 10648-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ERAB antibody (10648-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of MCF-7 using 10648-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ERAB antibody (10648-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).
FC experiment of MCF-7 using 10648-1-AP
1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ERAB Polyclonal antibody (10648-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

ERAB Polyclonal antibody

ERAB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10648-1-AP

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Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

HSD17B10, 3-hydroxyacyl-CoA dehydrogenase type II, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, 3alpha(or 20beta)-hydroxysteroid dehydrogenase, 3-alpha-(17-beta)-hydroxysteroid dehydrogenase

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

Size:

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概述

经过测试的应用
产品信息
发表文章 (7)
实验方案

Print datasheet

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, human brain tissue, HEK-293 cells, A2780 cells, mouse liver tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells
Positive FC (Intra) detected in	MCF-7 cells

Planning an IHC experiment? We recommend our IHCeasy HSD17B10 Ready-To-Use IHC Kit. HSD17B10 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 7 publications below
IP	See 1 publications below

产品信息

10648-1-AP targets ERAB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, IP, ELISA Application Description
Cited Applications	WB, IP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Immunogen	ERAB fusion protein Ag1020 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	hydroxysteroid (17-beta) dehydrogenase 10
Synonyms	HSD17B10, 3-hydroxyacyl-CoA dehydrogenase type II, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, 3alpha(or 20beta)-hydroxysteroid dehydrogenase, 3-alpha-(17-beta)-hydroxysteroid dehydrogenase
Calculated Molecular Weight	26 kDa
Observed Molecular Weight	27 kDa
GenBank Accession Number	BC008708
Gene Symbol	ERAB
Gene ID (NCBI)	3028
RRID	AB_2264270
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q99714
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

HSD17B10 (3-hydroxyacyl-CoA dehydrogenase type-2) is a multifunctional mitochondrial enzyme that acts on a wide spectrum of substrates, including neuroactive steroids, alcohols, leucine, and fatty acids, with a preference for short-chain methyl-branched acyl-CoAs(PMID:15860413).It has 2 isoforms produced by alternative splicing.Defects in HSD17B10 are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD deficiency) and mental retardation syndromic X-linked type 10 (MRXS10).

实验方案

Product Specific Protocols
WB protocol for ERAB antibody 10648-1-AP	Download protocol
IHC protocol for ERAB antibody 10648-1-AP	Download protocol
IF protocol for ERAB antibody 10648-1-AP	Download protocol
IP protocol for ERAB antibody 10648-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Cell Rep Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly. Authors - Oliver Rackham View Article
human	WB	Hum Mol Genet Leber's hereditary optic neuropathy (LHON)-associated ND6 14 484 T > C mutation caused pleiotropic effects on the complex I, RNA homeostasis, apoptosis and mitophagy. Authors - Min Liang View Article
mouse	WB	Endocrinology Wnt16 Promotes Vascular Smooth Muscle Contractile Phenotype and Function via Taz (Wwtr1) Activation in Male LDLR-/- Mice Authors - Abraham Behrmann View Article
rat	WB	J Oleo Sci Effects of Dietary Fat Restriction on Endurance Training-induced Metabolic Adaptations in Rat Skeletal Muscle. Authors - Takuya Karasawa View Article
mouse	WB	Circulation Stimulates Short-Chain Dehydrogenase/Reductase Proteins to Alleviate Heart Failure Independent of Mitochondrial Protein Deacetylation Authors - Matthew A Walker View Article
rat	WB	Gen Comp Endocrinol Testosterone regulates thymic remodeling by altering metabolic reprogramming in male rats Authors - Dong Li View Article

验证数据展示

WB analysis of HEK-293 using 10648-1-AP

WB analysis of HeLa using 10648-1-AP

IHC staining of human breast cancer using 10648-1-AP

IP experiment of HeLa using 10648-1-AP

IHC staining of mouse brain using 10648-1-AP

IF Staining of MCF-7 using 10648-1-AP

IF Staining of MCF-7 using 10648-1-AP

FC experiment of MCF-7 using 10648-1-AP

ERAB Polyclonal antibody

ERAB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

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经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly.

Leber's hereditary optic neuropathy (LHON)-associated ND6 14 484 T > C mutation caused pleiotropic effects on the complex I, RNA homeostasis, apoptosis and mitophagy.

Wnt16 Promotes Vascular Smooth Muscle Contractile Phenotype and Function via Taz (Wwtr1) Activation in Male LDLR-/- Mice

Effects of Dietary Fat Restriction on Endurance Training-induced Metabolic Adaptations in Rat Skeletal Muscle.

Stimulates Short-Chain Dehydrogenase/Reductase Proteins to Alleviate Heart Failure Independent of Mitochondrial Protein Deacetylation

Testosterone regulates thymic remodeling by altering metabolic reprogramming in male rats