验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, mouse brain tissue, mouse heart tissue, HepG2 cells, rat liver tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human prostate cancer tissue, mouse liver tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 16 publications below |
产品信息
15116-1-AP targets HSD17B4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, sheep |
| 免疫原 | HSD17B4 fusion protein Ag7165 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hydroxysteroid (17-beta) dehydrogenase 4 |
| 别名 | D-bifunctional protein, D bifunctional protein, 17-beta-hydroxysteroid dehydrogenase 4, 17-beta-HSD 4, 17 beta HSD 4 |
| 计算分子量 | 80 kDa |
| 观测分子量 | 80 kDa, 45 kDa |
| GenBank蛋白编号 | BC003098 |
| 基因名称 | HSD17B4 |
| Gene ID (NCBI) | 3295 |
| RRID | AB_2119959 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P51659 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSD17B4 (17-beta-hydroxysteroid dehydrogenase 4) is also named as Peroxisomal multifunctional enzyme type 2, D-bifuntional protein or multifunctional protein 2. It codes for a 80 kDa enzyme containing three distinct functional domains and is localized in peroxisomes. It is a bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids and catalyzing the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-brancked-chian fatty acids. After peroxisomal import, the full-length protein is proteolytically cleaved to yield a 35-kDa dehydrogenase subunit and a 45-kDa hydratase subunit containing the hydratase and SCP domains (PMID: 28868548, 24602372).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HSD17B4 antibody 15116-1-AP | Download protocol |
| IHC protocol for HSD17B4 antibody 15116-1-AP | Download protocol |
| IF protocol for HSD17B4 antibody 15116-1-AP | Download protocol |
| IP protocol for HSD17B4 antibody 15116-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Commun Biol Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism | ||
Neurobiol Dis Peroxisomal multifunctional protein-2 deficiency causes neuroinflammation and degeneration of Purkinje cells independent of very long chain fatty acid accumulation. | ||
Sci Rep Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells. | ||
Cells Cell Type-Selective Loss of Peroxisomal β-Oxidation Impairs Bipolar Cell but Not Photoreceptor Survival in the Retina. | ||
Mol Cells The Peroxisomal Localization of Hsd17b4 Is Regulated by Its Interaction with Phosphatidylserine. | ||
Biology (Basel) In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome. |
