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HSD17B7 Polyclonal antibody

HSD17B7 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16925-1-AP

Print datasheet

Synonyms

Hsd17b_2, HSD17B7, HSD17B7P2

验证数据展示

  • WB analysis of human liver using 16925-1-AP

    WB analysis of human liver using 16925-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16925-1-AP (HSD17B7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 16925-1-AP

    WB analysis of HEK-293 using 16925-1-AP

    WB result of HSD17B7 antibody (16925-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSD17B7 transfected HEK-293 cells.

  • IHC staining of human liver using 16925-1-AP

    IHC staining of human liver using 16925-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16925-1-AP (HSD17B7 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 16925-1-AP

    IHC staining of human liver using 16925-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16925-1-AP (HSD17B7 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of L02 using 16925-1-AP

    IP experiment of L02 using 16925-1-AP

    IP result of anti-HSD17B7 (IP:16925-1-AP, 4ug; Detection:16925-1-AP 1:500) with L02 cells lysate 2800ug.

经过测试的应用

Positive WB detected inhuman liver tissue, L02 cells, HEK-293 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16925-1-AP targets HSD17B7 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human
Immunogen HSD17B7 fusion protein Ag10315 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hydroxysteroid (17-beta) dehydrogenase 7 pseudogene 2
Synonyms Hsd17b_2, HSD17B7, HSD17B7P2
Calculated Molecular Weight 26 kDa, 37 kDa
Observed Molecular Weight 32-37 kDa
GenBank Accession NumberBC071987
Gene Symbol HSD17B7
Gene ID (NCBI) 158160
RRIDAB_2119975
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HSD17B7 antibody 16925-1-APDownload protocol
IHC protocol for HSD17B7 antibody 16925-1-APDownload protocol
IP protocol for HSD17B7 antibody 16925-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols