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验证数据展示

  • WB analysis of human liver using 16925-1-AP

    WB analysis of human liver using 16925-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16925-1-AP (HSD17B7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 16925-1-AP

    WB analysis of HEK-293 using 16925-1-AP

    WB result of HSD17B7 antibody (16925-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSD17B7 transfected HEK-293 cells.

  • IHC staining of human liver using 16925-1-AP

    IHC staining of human liver using 16925-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16925-1-AP (HSD17B7 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 16925-1-AP

    IHC staining of human liver using 16925-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16925-1-AP (HSD17B7 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of L02 using 16925-1-AP

    IP experiment of L02 using 16925-1-AP

    IP result of anti-HSD17B7 (IP:16925-1-AP, 4ug; Detection:16925-1-AP 1:500) with L02 cells lysate 2800ug.

  • Featured Product
  • KD/KO验证

HSD17B7 Polyclonal antibody

HSD17B7 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 16925-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, IHC, ELISA

别名

Hsd17b_2, HSD17B7, HSD17B7P2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman liver tissue, L02 cells, HEK-293 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16925-1-AP targets HSD17B7 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human
免疫原 HSD17B7 fusion protein Ag10315 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hydroxysteroid (17-beta) dehydrogenase 7 pseudogene 2
别名 Hsd17b_2, HSD17B7, HSD17B7P2
计算分子量 26 kDa, 37 kDa
观测分子量 32-37 kDa
GenBank蛋白编号BC071987
基因名称 HSD17B7
Gene ID (NCBI) 158160
RRIDAB_2119975
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HSD17B7 antibody 16925-1-APDownload protocol
IHC protocol for HSD17B7 antibody 16925-1-APDownload protocol
IP protocol for HSD17B7 antibody 16925-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols