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验证数据展示

  • WB analysis using 16752-1-AP

    WB analysis using 16752-1-AP

    Hela cells lysates were subjected to SDS PAGE followed by western blot with 16752-1-AP (HSD17B8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 16752-1-AP

    IHC staining of human liver cancer using 16752-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16752-1-AP (HSD17B8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 16752-1-AP

    IHC staining of human liver cancer using 16752-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16752-1-AP (HSD17B8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HSD17B8 Polyclonal antibody

HSD17B8 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16752-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

17 beta HSD 8, D6S2245E, FABG, FABGL, H2 KE6, HKE6, HSD17B8, Ke 6, KE6, Protein Ke6, RING2, SDR30C1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse liver tissue, mouse small intestine tissue, human testis tissue
Positive IHC detected inhuman liver cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16752-1-AP targets HSD17B8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen HSD17B8 fusion protein Ag10226 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hydroxysteroid (17-beta) dehydrogenase 8
Synonyms 17 beta HSD 8, D6S2245E, FABG, FABGL, H2 KE6, HKE6, HSD17B8, Ke 6, KE6, Protein Ke6, RING2, SDR30C1
Calculated Molecular Weight 261 aa, 27 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC008185
Gene Symbol HSD17B8
Gene ID (NCBI) 7923
RRIDAB_2248540
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92506
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HSD17B8 antibody 16752-1-APDownload protocol
IHC protocol for HSD17B8 antibody 16752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Cell facilitation promotes growth and survival under drug pressure in breast cancer

Authors - Rena Emond
View Article