HSD3B2 Polyclonal antibody
HSD3B2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IHC detected in | rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15516-1-AP targets HSD3B2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, canine |
| Immunogen | HSD3B2 fusion protein Ag7782 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 |
| Synonyms | 3b HSD, HSD3B2, HSD3β2, HSDB, HSDB3, HSDB3B, SDR11E2 |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC038419 |
| Gene Symbol | HSD3B2 |
| Gene ID (NCBI) | 3284 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P26439 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSD3B2(3-beta-hydroxy-Delta(5)-steroid dehydrogenase) belongs to the 3-beta-HSD family and catalyzes the oxidation and isomerization of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, thus leading to the formation of all classes of steroid hormones.HSD3B2 is expressed almost exclusively in adrenals and gonads, whereas HSD3B1 is expressed predominantly in placenta and skin (PMID:1682237). HSD3B2 plays a crucial role in steroid hormone biosynthesis and is thus of particular interest in hormone dependent tumors such as prostate cancer. Compared with normal tissue cytoplasmic HSD3B2 staining was stronger in prostate cancers (PMID: 29803408). Defects in HSD3B2 are the cause of adrenal hyperplasia type 2 (AH2).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HSD3B2 antibody 15516-1-AP | Download protocol |
| IHC protocol for HSD3B2 antibody 15516-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Hazard Mater Tributyltin chloride (TBTCL) induces cell injury via dysregulation of endoplasmic reticulum stress and autophagy in Leydig cells | ||
Oncotarget Generation and characteristics of human Sertoli cell line immortalized by overexpression of human telomerase. | ||
Endocrinology A dietary medium-chain fatty acid, decanoic acid, inhibits recruitment of Nur77 to the HSD3B2 promoter in vitro, and reverses endocrine and metabolic abnormalities in a rat model of polycystic ovary syndrome. | ||
Front Physiol Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation. | ||
Reprod Biomed Online Brain-derived neurotrophic factor expression in granulosa lutein cells. | ||
Int J Clin Exp Pathol Ectopic cortisol-producing adrenocortical adenoma in the renal hilum: histopathological features and steroidogenic enzyme profile. |