• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of mouse liver using 10488-1-AP

    WB analysis of mouse liver using 10488-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10488-1-AP (HSD3B7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 10488-1-AP

    IHC staining of human pancreas cancer using 10488-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10488-1-AP (HSD3B7 antibody) at dilution of 1:50 (under 10x lens).

  • IP experiment of mouse liver using 10488-1-AP

    IP experiment of mouse liver using 10488-1-AP

    IP result of anti-HSD3B7 (IP:10488-1-AP, 4ug; Detection:10488-1-AP 1:300) with mouse liver tissue lysate 6000 ug.

HSD3B7 Polyclonal antibody

HSD3B7 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 10488-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

3 beta HSD VII, C(27) 3 beta HSD, HSD3B7, PFIC4, SDR11E3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman pancreas cancer tissue, human kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10488-1-AP targets HSD3B7 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 HSD3B7 fusion protein Ag0755 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7
别名 3 beta HSD VII, C(27) 3 beta HSD, HSD3B7, PFIC4, SDR11E3
计算分子量 41 kDa
观测分子量 36 kDa
GenBank蛋白编号BC004929
基因名称 HSD3B7
Gene ID (NCBI) 80270
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H2F3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSD3B7(3 beta-hydroxysteroid dehydrogenase type 7) is also named as 3-beta-HSD VII,C(27) 3-beta-HSD and belongs to the 3-beta-HSD family. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids and HSD VII is active against four 7-alpha-hydroxylated sterols. This enzyme is expressed predominantly in the liver(PMID:11067870). Defects in HSD3B7 are the cause of congenital bile acid synthesis defect type 1 (CBAS1). It has 2 isoforms produced by alternative splicing.