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HSDL1 Polyclonal antibody

HSDL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16988-1-AP

Print datasheet

Synonyms

HSDL1, SDR12C3

验证数据展示

  • WB analysis using 16988-1-AP

    WB analysis using 16988-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16988-1-AP (HSDL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 16988-1-AP

    IHC staining of human breast cancer using 16988-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16988-1-AP (HSDL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 16988-1-AP

    IHC staining of human breast cancer using 16988-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16988-1-AP (HSDL1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, fetal human brain tissue
Positive IHC detected inhuman breast cancer tissue, human kidney tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16988-1-AP targets HSDL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Immunogen HSDL1 fusion protein Ag10588 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hydroxysteroid dehydrogenase like 1
Synonyms HSDL1, SDR12C3
Calculated Molecular Weight 330 aa, 37 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC018084
Gene Symbol HSDL1
Gene ID (NCBI) 83693
RRIDAB_2120235
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3SXM5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HSDL1 antibody 16988-1-APDownload protocol
IHC protocol for HSDL1 antibody 16988-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Sci Adv

Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.

Authors - Odetta Antico
View Article