验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, mouse spleen tissue, mouse testis tissue, HepG2 cells, MCF-7 cells, mouse kidney tissue |
| Positive IHC detected in | human cervical cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16107-1-AP targets HSF1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | HSF1 fusion protein Ag9023 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | heat shock transcription factor 1 |
| Synonyms | Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1 |
| Calculated Molecular Weight | 529 aa, 57 kDa |
| Observed Molecular Weight | 68-80 kDa |
| GenBank Accession Number | BC014638 |
| Gene Symbol | HSF1 |
| Gene ID (NCBI) | 3297 |
| RRID | AB_11182610 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q00613 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HSF1 antibody 16107-1-AP | Download protocol |
| IHC protocol for HSF1 antibody 16107-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Commun RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases. | ||
Am J Physiol Cell Physiol Cancer associated fibroblasts suppressed ferroptosis in glioblastoma via upregulating lncRNA DLEU1 | ||
Ann Thorac Surg Upregulation of Nuclear Heat Shock Factor 1 Contributes to Tumor Angiogenesis and Poor Survival in Patients With Non-Small Cell Lung Cancer. | ||
Front Microbiol The Capsid Protein VP1 of Coxsackievirus B Induces Cell Cycle Arrest by Up-Regulating Heat Shock Protein 70. | ||
Pharm Biol Aspirin relieves the calcification of aortic smooth muscle cells by enhancing the heat shock response. | ||
Toxicol In Vitro Ginsenoside Rg3 induces FUT4-mediated apoptosis in H. pylori CagA-treated gastric cancer cells by regulating SP1 and HSF1 expressions. |
