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HSF1 Polyclonal antibody

HSF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 51034-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, A431 cells, HepG2 cells, K-562 cells, NIH/3T3 cells, HEK-293T cells, C6 cells, mouse ovary tissue, mouse testis tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman lung cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy HSF1 Ready-To-Use IHC Kit. HSF1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51034-1-AP targets HSF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, bovine
免疫原 HSF1 fusion protein Ag0739 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heat shock transcription factor 1
别名 Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1
计算分子量 529 aa, 57 kDa
观测分子量 68-80 kDa
GenBank蛋白编号BC014638
基因名称 HSF1
Gene ID (NCBI) 3297
RRIDAB_2120269
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ00613
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).

实验方案

Product Specific Protocols
WB protocol for HSF1 antibody 51034-1-APDownload protocol
IHC protocol for HSF1 antibody 51034-1-APDownload protocol
IF protocol for HSF1 antibody 51034-1-APDownload protocol
IP protocol for HSF1 antibody 51034-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Science

Z-DNA binding protein 1 promotes heatstroke-induced cell death.

Authors - Fangfang Yuan
mouseWB

Nat Commun

Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagy

Authors - Donghai Cui
ratWB

Dev Cell

Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination

Authors - Li Li
mouseWB

Food Funct

Protective effects of L-theanine and dihydromyricetin on reproductive function in male mice under heat stress

Authors - Difei Yang
rat

Int J Pharm

Effective myocardial infarction treatment by targeted accumulation of Sulforaphane using porous magnetic silica nanoparticles

Authors - Jian Zhang
humanWB

Am J Physiol Cell Physiol

Cancer associated fibroblasts suppressed ferroptosis in glioblastoma via upregulating lncRNA DLEU1

Authors - Jie Zhao
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