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  • Featured Product
  • KD/KO Validated

HSF2 Polyclonal antibody

HSF2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10706-1-AP

Print datasheet

Synonyms

Heat shock factor protein 2, HSF 2, HSF2, HSTF 2, HSTF2

验证数据展示

  • WB analysis using 10706-1-AP

    WB analysis using 10706-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10706-1-AP (HSF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 10706-1-AP

    IHC staining of mouse testis using 10706-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10706-1-AP (HSF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 10706-1-AP

    IHC staining of mouse testis using 10706-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10706-1-AP (HSF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 10706-1-AP

    IHC staining of rat testis using 10706-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 10706-1-AP (HSF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 10706-1-AP

    IHC staining of rat testis using 10706-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 10706-1-AP (HSF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, A549 cells
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10706-1-AP targets HSF2 in WB, IHC, ELISA applications and shows reactivity with human, Mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, Mouse, rat
Cited Reactivityhuman
Immunogen HSF2 fusion protein Ag1067 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heat shock transcription factor 2
Synonyms Heat shock factor protein 2, HSF 2, HSF2, HSTF 2, HSTF2
Calculated Molecular Weight 79 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC005329
Gene Symbol HSF2
Gene ID (NCBI) 3298
RRIDAB_2120443
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ03933
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HSF2 antibody 10706-1-APDownload protocol
IHC protocol for HSF2 antibody 10706-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Am J Cancer Res

HSF2 regulates aerobic glycolysis by suppression of FBP1 in hepatocellular carcinoma.

Authors - Li-Na Yang
  • KD Validated
View Article