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验证数据展示

  • WB analysis using 10706-1-AP

    WB analysis using 10706-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10706-1-AP (HSF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 10706-1-AP

    IHC staining of mouse testis using 10706-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10706-1-AP (HSF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 10706-1-AP

    IHC staining of mouse testis using 10706-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10706-1-AP (HSF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 10706-1-AP

    IHC staining of rat testis using 10706-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 10706-1-AP (HSF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 10706-1-AP

    IHC staining of rat testis using 10706-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 10706-1-AP (HSF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

HSF2 Polyclonal antibody

HSF2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10706-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, Mouse, rat

应用

WB, IHC, ELISA

别名

Heat shock factor protein 2, HSF 2, HSF2, HSTF 2, HSTF2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, A549 cells
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10706-1-AP targets HSF2 in WB, IHC, ELISA applications and shows reactivity with human, Mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, Mouse, rat
文献引用反应性human
免疫原 HSF2 fusion protein Ag1067 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heat shock transcription factor 2
别名 Heat shock factor protein 2, HSF 2, HSF2, HSTF 2, HSTF2
计算分子量 79 kDa
观测分子量 60 kDa
GenBank蛋白编号BC005329
基因名称 HSF2
Gene ID (NCBI) 3298
RRIDAB_2120443
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ03933
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HSF2 antibody 10706-1-APDownload protocol
IHC protocol for HSF2 antibody 10706-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Am J Cancer Res

HSF2 regulates aerobic glycolysis by suppression of FBP1 in hepatocellular carcinoma.

Authors - Li-Na Yang
  • KD Validated
View Article