验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse skeletal muscle tissue, human skeletal muscle tissue, human heart tissue, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
产品信息
18797-1-AP targets HSF4 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | heat shock transcription factor 4 |
| 别名 | CTM, Heat shock factor protein 4, hHSF4, HSF 4, HSF4, HSTF 4 |
| 计算分子量 | 53 kDa |
| 观测分子量 | 53-58 kDa |
| GenBank蛋白编号 | BC130383 |
| 基因名称 | HSF4 |
| Gene ID (NCBI) | 3299 |
| RRID | AB_10598014 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9ULV5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSF4 belongs to the HSF family. HSF4 is a DNA-binding protein that specifically binds heat shock promoter elements (HSE). The isoform HSF4A represses transcription while the isoform HSF4B activates transcription. HSF4 is a homotrimer which exhibits constitutive DNA binding and forms trimers even in the absence of stress. It is expressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues. Mutation of HSF4 will cause lamellar cataract which is known as Marner type cataract (CAM). Lamellar cataract is an autosomal dominant common type of infantile cataract. Finger malformation is observed in some kindreds. Inheritance of these forms of cataract is autosomal dominant.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HSF4 antibody 18797-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene FGD3 binds with HSF4 to suppress p65 expression and inhibit pancreatic cancer progression.
| ||
Mol Cell Biochem HSF4 promotes tumor progression of colorectal cancer by transactivating c-MET
| ||
Invest Ophthalmol Vis Sci HSF4 Transcriptionally Activates Autophagy by Regulating ATG9a During Lens Terminal Differentiation |
