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HSFY1 Polyclonal antibody

HSFY1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11970-1-AP

Print datasheet

Synonyms

HSF2 like, HSF2L, HSFY, HSFY1, HSFY2

验证数据展示

  • WB analysis of human testis using 11970-1-AP

    WB analysis of human testis using 11970-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 11970-1-AP (HSFY1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 11970-1-AP

    IHC staining of human testis using 11970-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11970-1-AP (HSFY1 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11970-1-AP targets HSFY1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen HSFY1 fusion protein Ag2592 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heat shock transcription factor, Y-linked 1
Synonyms HSF2 like, HSF2L, HSFY, HSFY1, HSFY2
Calculated Molecular Weight 401 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC055414
Gene Symbol HSFY1
Gene ID (NCBI) 86614
RRIDAB_2877810
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96LI6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The HSFY gene is located in the male-specific region of chromosome Y (MSY), which is not involved in X-Y crossover events. HSFY gene is expressed exclusively in testis. HSFY is a DNA-binding protein that specifically binds heat shock promoter elements(HSE), and suggested to be implicated in spermatogenesis both in animals and humans.

实验方案

Product Specific Protocols
WB protocol for HSFY1 antibody 11970-1-APDownload protocol
IHC protocol for HSFY1 antibody 11970-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols