验证数据展示
经过测试的应用
| Positive WB detected in | pig skeletal muscle tissue, mouse heart tissue, rat heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive IF/ICC detected in | U-118 MG cells |
Planning an IHC experiment? We recommend our IHCeasy HSPB6 Ready-To-Use IHC Kit. HSPB6 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:850-1:3400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
67327-1-Ig targets HSP20 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human |
| 免疫原 | HSP20 fusion protein Ag10484 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | heat shock protein, alpha-crystallin-related, B6 |
| 别名 | HspB6, Heat shock protein family B member 6, Heat shock protein beta-6, Heat shock protein beta 6, Heat shock 20 kDa-like protein p20 |
| 计算分子量 | 160 aa, 17 kDa |
| 观测分子量 | 17 kDa |
| GenBank蛋白编号 | BC068046 |
| 基因名称 | HSP20 |
| Gene ID (NCBI) | 126393 |
| RRID | AB_2882586 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O14558 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hsp20 is a member of the small heat shock superfamily of proteins that function as chaperones to prevent protein misfolding through adenosine triphosphate−independent processes. It is widely expressed but is most abundant in cardiac, skeletal, and smooth muscles. Phosphorylation of HSP20 at Ser16 was elevated in human failing hearts as well as in murine hearts after I/R injury Indicating its cardioprotective function.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HSP20 antibody 67327-1-Ig | Download protocol |
| IHC protocol for HSP20 antibody 67327-1-Ig | Download protocol |
| IF protocol for HSP20 antibody 67327-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |