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  • Featured Product
  • KD/KO Validated

HSPA4 Polyclonal antibody

HSPA4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 21206-1-AP

Print datasheet

Synonyms

HSPH2, hsp70RY, Heat shock protein family H member 2, Heat shock 70-related protein APG-2, Heat shock 70 kDa protein 4

验证数据展示

  • WB analysis using 21206-1-AP

    WB analysis using 21206-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21206-1-AP (HSPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 21206-1-AP

    IHC staining of human colon cancer using 21206-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human colon cancer using 21206-1-AP

    IHC staining of human colon cancer using 21206-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 21206-1-AP

    IF Staining of HepG2 using 21206-1-AP

    Immunofluorescent analysis of HepG2 cells, using HSPA4 antibody 21206-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of mouse brain using 21206-1-AP

    IP experiment of mouse brain using 21206-1-AP

    IP result of anti-HSPA4 (IP:21206-1-AP, 4ug; Detection:21206-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • WB analysis of HEK-293 using 21206-1-AP

    WB analysis of HEK-293 using 21206-1-AP

    WB result of HSPA4 antibody (21206-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPA4 transfected HEK-293 cells.

  • IF Staining of MCF-7 using 21206-1-AP

    IF Staining of MCF-7 using 21206-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HSPA4 antibody (21206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of C6 using 21206-1-AP

    FC experiment of C6 using 21206-1-AP

    1x10^6 C6 cells were intracellularly stained with 0.4 ug HSPA4 Polyclonal antibody (21206-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inC6 cells, HEK-293 cells, mouse testis tissue, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HepG2 cells
Positive FC (Intra) detected inC6 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21206-1-AP targets HSPA4 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, ELISA
Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse
Immunogen HSPA4 fusion protein Ag15581 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heat shock 70kDa protein 4
Synonyms HSPH2, hsp70RY, Heat shock protein family H member 2, Heat shock 70-related protein APG-2, Heat shock 70 kDa protein 4
Calculated Molecular Weight 840 aa, 94 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC110861
Gene Symbol HSPA4
Gene ID (NCBI) 3308
RRIDAB_10733641
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP34932
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSPA4 (also known as Apg-2, HSP70RY) is a 110 kDa cytosolic protein, a member of the heat-shock protein 110 (HSP110) subfamily of HSP70 proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA4 is ubiquitously expressed and its expression is not heat inducible. Overexpression of HSPA4 has been reported in some leukemia and solid tumors. This antibody well recognized the endogenous HSPA4 protein in mouse brain/ testis tissues. (PMID: 21487003)

实验方案

Product Specific Protocols
WB protocol for HSPA4 antibody 21206-1-APDownload protocol
IHC protocol for HSPA4 antibody 21206-1-APDownload protocol
IF protocol for HSPA4 antibody 21206-1-APDownload protocol
IP protocol for HSPA4 antibody 21206-1-APDownload protocol
FC protocol for HSPA4 antibody 21206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Biochem Cell Biol

Folate deprivation induces cell cycle arrest at G0/G1 phase and apoptosis in hippocampal neuron cells through down-regulation of IGF-1 signaling pathway.

Authors - Yang Yang
View Article
humanELISA

Eur J Clin Invest

Salivary monitoring related to major surgery.

Authors - Ullmann Yehuda Y
View Article
mouseWB

Int J Med Sci

Exercise combined with trimetazidine improves anti-fatal stress capacity through enhancing autophagy and heat shock protein 70 of myocardium in mice.

Authors - Lingjun Jiang
View Article
humanWB

Mol Cell Proteomics

A new cellular interactome of SARS-CoV-2 nucleocapsid protein and its biological implications

Authors - Yuan-Qin Min
View Article