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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 80735-1-RR (same clone as 80735-1-PBS)

    WB analysis using 80735-1-RR (same clone as 80735-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80735-1-RR (HSP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80735-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 80735-1-RR (same clone as 80735-1-PBS)

    IHC staining of human breast cancer using 80735-1-RR (same clone as 80735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80735-1-RR (HSP70 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80735-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSP70 antibody included
  • IF Staining of HeLa using 80735-1-RR (same clone as 80735-1-PBS)

    IF Staining of HeLa using 80735-1-RR (same clone as 80735-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HSP70 antibody (80735-1-RR, Clone: 4E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80735-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 80735-1-RR (same clone as 80735-1-PBS)

    IHC staining of human colon cancer using 80735-1-RR (same clone as 80735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80735-1-RR (HSP70 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80735-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSP70 antibody included
  • IHC staining of human lung squamous cell carcinoma using 80735-1-RR (same clone as 80735-1-PBS)

    IHC staining of human lung squamous cell carcinoma using 80735-1-RR (same clone as 80735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 80735-1-RR (HSP70 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80735-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSP70 antibody included

HSP70 Recombinant antibody, PBS Only

HSP70 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 80735-1-PBS

产品说明书

CloneNo. 4E10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, yeast

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

HSPA1A, HSP72, HSP70.1, Heat shock protein family A member 1A, heat shock 70kDa protein 1A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HSP70 Ready-To-Use IHC Kit. HSP70 primary antibody included.

产品信息

80735-1-PBS targets HSP70 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, yeast samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, yeast
免疫原 HSP70 fusion protein Ag1446 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heat shock 70kDa protein 1A
别名 HSPA1A, HSP72, HSP70.1, Heat shock protein family A member 1A, heat shock 70kDa protein 1A
计算分子量 70 kDa
观测分子量70 kDa
GenBank蛋白编号BC009322
基因名称 HSP70
Gene ID (NCBI) 3303
RRIDAB_2918908
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP0DMV8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSPA1A , collectively known as HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is also used as exosomal marker