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HSP90 Recombinant antibody

HSP90 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241050A10

Cat no : 83950-5-RR

Print datasheet

Synonyms

HSP90AA1, HSP90A, Heat shock protein HSP 90-alpha, Heat shock protein HSP 90 alpha, Heat shock protein family C member 1

验证数据展示

  • WB analysis using 83950-5-RR

    WB analysis using 83950-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83950-5-RR (HSP90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 83950-5-RR

    IF Staining of HepG2 using 83950-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP90 antibody (83950-5-RR, Clone: 241050A10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of Jurkat using 83950-5-RR

    FC experiment of Jurkat using 83950-5-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug HSP90 Recombinant antibody (83950-5-RR, Clone:241050A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83950-5-RR

    Affinity and Kinetic Characterization of 83950-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83950-5-RR against Human HSP90 were performed. The affinity constant is 0.692 nM.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, LNCaP cells, NIH/3T3 cells, C6 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy HSP90AA1 Ready-To-Use IHC Kit. HSP90AA1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83950-5-RR targets HSP90 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen HSP90 fusion protein Ag3826 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name heat shock protein 90kDa alpha (cytosolic), class A member 1
Synonyms HSP90AA1, HSP90A, Heat shock protein HSP 90-alpha, Heat shock protein HSP 90 alpha, Heat shock protein family C member 1
Calculated Molecular Weight 853 aa, 90 kDa
GenBank Accession NumberBC023006
Gene Symbol HSP90
Gene ID (NCBI) 3320
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP07900
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.

实验方案

Product Specific Protocols
WB protocol for HSP90 antibody 83950-5-RRDownload protocol
IF protocol for HSP90 antibody 83950-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols