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  • Featured Product
  • KD/KO Validated

HSP90AB1 Recombinant antibody, PBS Only

HSP90AB1 Recombinant Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, zebrafish

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

5P14

Cat No : 80301-1-PBS

Print datasheet

Synonyms

D6S182, FLJ26984, Heat shock 84 kDa, Heat shock protein HSP 90 beta, HS90B, HSP 84, HSP 90, HSP84, HSP90 BETA, HSP90AB1, HSP90B, HSPC2, HSPCB

验证数据展示

  • IHC staining of human breast cancer using 80301-1-RR (same clone as 80301-1-PBS)

    IHC staining of human breast cancer using 80301-1-RR (same clone as 80301-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80301-1-RR (HSP90AB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSP90AB1 antibody included
  • IF Staining of HeLa using 80301-1-RR (same clone as 80301-1-PBS)

    IF Staining of HeLa using 80301-1-RR (same clone as 80301-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HSP90AB1 antibody (80301-1-RR, Clone: 5P14 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

  • WB analysis using 80301-1-RR (same clone as 80301-1-PBS)

    WB analysis using 80301-1-RR (same clone as 80301-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80301-1-RR (HSP90AB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 80301-1-RR (same clone as 80301-1-PBS)

    WB analysis of HEK-293 using 80301-1-RR (same clone as 80301-1-PBS)

    WB result of HSP90AB1 antibody (80301-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP90AB1 transfected HEK-293 cells. This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HSP90AB1 Ready-To-Use IHC Kit. HSP90AB1 primary antibody included.

产品信息

80301-1-PBS targets HSP90AB1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, zebrafish samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, mouse, rat, zebrafish
Immunogen HSP90AB1 fusion protein Ag1947 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name heat shock protein 90kDa alpha (cytosolic), class B member 1
Synonyms D6S182, FLJ26984, Heat shock 84 kDa, Heat shock protein HSP 90 beta, HS90B, HSP 84, HSP 90, HSP84, HSP90 BETA, HSP90AB1, HSP90B, HSPC2, HSPCB
Calculated Molecular Weight 723 aa, 83 kDa
GenBank Accession NumberBC014485
Gene Symbol HSP90AB1
Gene ID (NCBI) 3326
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08238
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers.