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HSPA13 Polyclonal antibody

HSPA13 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 12667-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA and More (2)

Heat shock 70 kDa protein 13, HSPA13, STCH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HSPA13 Ready-To-Use IHC Kit. HSPA13 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12667-2-AP targets HSPA13 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 HSPA13 fusion protein Ag3349 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heat shock protein 70kDa family, member 13
别名 Heat shock 70 kDa protein 13, HSPA13, STCH
计算分子量 471 aa, 52 kDa
观测分子量 60, 70 kDa
GenBank蛋白编号BC036370
基因名称 HSPA13
Gene ID (NCBI) 6782
RRIDAB_2119204
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48723
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSPA13 (Heat shock 70 kDa protein 13), also known as STCH (the stress 70 protein chaperone), is a member of the Hsp70 family of ATPase "stress" or "heat shock" proteins. Human STCH encodes a 60 kDa protein that is highly enriched in the ER. Recently it has been reported that overexpression of the HSPA13 gene reduces prion disease incubation time in mice.

实验方案

Product Specific Protocols
WB protocol for HSPA13 antibody 12667-2-APDownload protocol
IHC protocol for HSPA13 antibody 12667-2-APDownload protocol
IP protocol for HSPA13 antibody 12667-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

HSPA13 facilitates NF-κB-mediated transcription and attenuates cell death responses in TNFα signaling.

Authors - Chun Gao
  • KO Validated
humanWB

Proc Natl Acad Sci U S A

Overexpression of the Hspa13 (Stch) gene reduces prion disease incubation time in mice.

Authors - Grizenkova Julia J
mouseWB,CoIP

Front Immunol

Hspa13 Promotes Plasma Cell Production and Antibody Secretion.

Authors - Youdi He
humanWB

Endocrinology

Reassessment of the Proteomic Composition and Function of Extracellular Vesicles in the Seminal Plasma.

Authors - Hanshu Wang
humanWB,IHC

Proteome Sci

PRM-based quantitative proteomics analysis of altered HSP abundance in villi and decidua of patients with early missed abortion

Authors - Xiao-Fang Chen
human,mouseWB,IHC,CoIP

Cell Death Discov

Heat shock protein HSPA13 promotes hepatocellular carcinoma progression by stabilizing TANK

Authors - Xuesong Cen
  • KD Validated
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