验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, K-562 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12667-2-AP targets HSPA13 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
文献引用应用 | WB, IHC, IF, CoIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | HSPA13 fusion protein Ag3349 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | heat shock protein 70kDa family, member 13 |
别名 | Heat shock 70 kDa protein 13, HSPA13, STCH |
计算分子量 | 471 aa, 52 kDa |
观测分子量 | 60, 70 kDa |
GenBank蛋白编号 | BC036370 |
基因名称 | HSPA13 |
Gene ID (NCBI) | 6782 |
RRID | AB_2119204 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P48723 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSPA13 (Heat shock 70 kDa protein 13), also known as STCH (the stress 70 protein chaperone), is a member of the Hsp70 family of ATPase "stress" or "heat shock" proteins. Human STCH encodes a 60 kDa protein that is highly enriched in the ER. Recently it has been reported that overexpression of the HSPA13 gene reduces prion disease incubation time in mice.
实验方案
Product Specific Protocols | |
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WB protocol for HSPA13 antibody 12667-2-AP | Download protocol |
IHC protocol for HSPA13 antibody 12667-2-AP | Download protocol |
IP protocol for HSPA13 antibody 12667-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Sci Adv HSPA13 facilitates NF-κB-mediated transcription and attenuates cell death responses in TNFα signaling.
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Proc Natl Acad Sci U S A Overexpression of the Hspa13 (Stch) gene reduces prion disease incubation time in mice. | ||
Endocrinology Reassessment of the Proteomic Composition and Function of Extracellular Vesicles in the Seminal Plasma. | ||
Proteome Sci PRM-based quantitative proteomics analysis of altered HSP abundance in villi and decidua of patients with early missed abortion | ||
Cell Death Discov Heat shock protein HSPA13 promotes hepatocellular carcinoma progression by stabilizing TANK
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