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验证数据展示

  • WB analysis using 84426-5-RR

    WB analysis using 84426-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84426-5-RR (HSPA13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84426-5-RR

    Affinity and Kinetic Characterization of 84426-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84426-5-RR against Human HSPA13 were performed. The affinity constant is 39.3 nM.

HSPA13 Recombinant antibody

HSPA13 Recombinant Antibody for WB, ELISA
Cat No. 84426-5-RR

产品说明书

CloneNo. 241572E4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Stress-70 protein chaperone microsome-associated 60 kDa protein, STCH, Microsomal stress-70 protein ATPase core, Heat shock protein family A member 13, Heat shock 70 kDa protein 13

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, fetal human brain tissue
Planning an IHC experiment? We recommend our IHCeasy HSPA13 Ready-To-Use IHC Kit. HSPA13 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84426-5-RR targets HSPA13 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 HSPA13 fusion protein Ag3349 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heat shock protein 70kDa family, member 13
别名 Stress-70 protein chaperone microsome-associated 60 kDa protein, STCH, Microsomal stress-70 protein ATPase core, Heat shock protein family A member 13, Heat shock 70 kDa protein 13
计算分子量 471 aa, 52 kDa
观测分子量60 kDa
GenBank蛋白编号BC036370
基因名称 HSPA13
Gene ID (NCBI) 6782
RRIDAB_3671953
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP48723
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSPA13 (Heat shock 70 kDa protein 13), also known as STCH (the stress 70 protein chaperone), is a member of the Hsp70 family of ATPase "stress" or "heat shock" proteins. Human STCH encodes a 60 kDa protein that is highly enriched in the ER. Recently it has been reported that overexpression of the HSPA13 gene reduces prion disease incubation time in mice.

实验方案

Product Specific Protocols
WB protocol for HSPA13 antibody 84426-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols