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验证数据展示

  • WB analysis using 28503-1-AP

    WB analysis using 28503-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28503-1-AP (HSPA14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of K-562 using 28503-1-AP

    IF Staining of K-562 using 28503-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using HSPA14 antibody (28503-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human ovary cancer using 28503-1-AP

    IHC staining of human ovary cancer using 28503-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28503-1-AP (HSPA14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HSPA14 Polyclonal antibody

HSPA14 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28503-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HSP70 4, HSP60, Heat shock protein HSP60, Heat shock protein family A member 14, heat shock 70kDa protein 14

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28503-1-AP targets HSPA14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen HSPA14 fusion protein Ag27240 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heat shock 70kDa protein 14
Synonyms HSP70 4, HSP60, Heat shock protein HSP60, Heat shock protein family A member 14, heat shock 70kDa protein 14
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC119690
Gene Symbol HSPA14
Gene ID (NCBI) 51182
RRIDAB_3086059
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ0VDF9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HSPA14 antibody 28503-1-APDownload protocol
IHC protocol for HSPA14 antibody 28503-1-APDownload protocol
IF protocol for HSPA14 antibody 28503-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols