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HSPA14 Polyclonal antibody

HSPA14 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28503-1-AP

Print datasheet

Synonyms

HSP70 4, HSP60, Heat shock protein HSP60, Heat shock protein family A member 14, heat shock 70kDa protein 14

验证数据展示

  • WB analysis using 28503-1-AP

    WB analysis using 28503-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28503-1-AP (HSPA14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of K-562 using 28503-1-AP

    IF Staining of K-562 using 28503-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using HSPA14 antibody (28503-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human ovary cancer using 28503-1-AP

    IHC staining of human ovary cancer using 28503-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28503-1-AP (HSPA14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28503-1-AP targets HSPA14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen HSPA14 fusion protein Ag27240 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heat shock 70kDa protein 14
Synonyms HSP70 4, HSP60, Heat shock protein HSP60, Heat shock protein family A member 14, heat shock 70kDa protein 14
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC119690
Gene Symbol HSPA14
Gene ID (NCBI) 51182
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ0VDF9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HSPA14 antibody 28503-1-APDownload protocol
IHC protocol for HSPA14 antibody 28503-1-APDownload protocol
IF protocol for HSPA14 antibody 28503-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols