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HSPA1L Monoclonal antibody, PBS Only

HSPA1L Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E7H2

Cat no : 66780-1-PBS

Print datasheet

Synonyms

Heat shock 70 kDa protein 1L, HSP70 1L, HSP70 HOM, HSP70T, HSPA1L, hum70t

验证数据展示

  • WB analysis of HeLa using 66780-1-Ig (same clone as 66780-1-PBS)

    WB analysis of HeLa using 66780-1-Ig (same clone as 66780-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 66780-1-Ig (same clone as 66780-1-PBS)

    IHC staining of mouse testis using 66780-1-Ig (same clone as 66780-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66780-1-Ig (HSPA1L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSPA1L antibody included
  • IF Staining of HeLa using 66780-1-Ig (same clone as 66780-1-PBS)

    IF Staining of HeLa using 66780-1-Ig (same clone as 66780-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66780-1-Ig (HSPA1L antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HSPA1L Ready-To-Use IHC Kit. HSPA1L primary antibody included.

产品信息

66780-1-PBS targets HSPA1L in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen HSPA1L fusion protein Ag5182 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name heat shock 70kDa protein 1-like
Synonyms Heat shock 70 kDa protein 1L, HSP70 1L, HSP70 HOM, HSP70T, HSPA1L, hum70t
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC034483
Gene Symbol HSPA1L
Gene ID (NCBI) 3305
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP34931
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSPA1L (Hsp70-like protein 1, also known as HSP70-hom) is a member of Hsp70 family of heat shock proteins. Recently it has been reported that HSPA1L can interact with TLR4, activate dendritic cells and induce Th1 Response. This antibody well recognizes the endogenous HSPA1L protein in multiple cell lines. (21730052)