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HSPA2 Monoclonal antibody, PBS Only

HSPA2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A4A9

Cat no : 66291-1-PBS

Print datasheet

Synonyms

Heat shock 70 kDa protein 2, heat shock 70kDa protein 2, HSP70 2, HSP70 3, HSPA2

验证数据展示

  • WB analysis using 66291-1-Ig (same clone as 66291-1-PBS)

    WB analysis using 66291-1-Ig (same clone as 66291-1-PBS)

    Various lysates (25 μg/lane) were subjected to SDS PAGE followed by western blot with 66291-1-Ig (HSPA2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66291-1-Ig (same clone as 66291-1-PBS)

    IHC staining of human prostate cancer using 66291-1-Ig (same clone as 66291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66291-1-Ig (HSPA2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSPA2 antibody included
  • IHC staining of human testis using 66291-1-Ig (same clone as 66291-1-PBS)

    IHC staining of human testis using 66291-1-Ig (same clone as 66291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66291-1-Ig (HSPA2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSPA2 antibody included
  • IHC staining of human testis using 66291-1-Ig (same clone as 66291-1-PBS)

    IHC staining of human testis using 66291-1-Ig (same clone as 66291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66291-1-Ig (HSPA2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSPA2 antibody included
Planning an IHC experiment? We recommend our IHCeasy HSPA2 Ready-To-Use IHC Kit. HSPA2 primary antibody included.

产品信息

66291-1-PBS targets HSPA2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, rat, mouse
Immunogen HSPA2 fusion protein Ag24228 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name heat shock 70kDa protein 2
Synonyms Heat shock 70 kDa protein 2, heat shock 70kDa protein 2, HSP70 2, HSP70 3, HSPA2
Calculated Molecular Weight 639 aa, 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC036107
Gene Symbol HSPA2
Gene ID (NCBI) 3306
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP54652
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)