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Host
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 66291-1-Ig (same clone as 66291-1-PBS)

    WB analysis using 66291-1-Ig (same clone as 66291-1-PBS)

    Various lysates (25 μg/lane) were subjected to SDS PAGE followed by western blot with 66291-1-Ig (HSPA2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66291-1-Ig (same clone as 66291-1-PBS)

    IHC staining of human prostate cancer using 66291-1-Ig (same clone as 66291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66291-1-Ig (HSPA2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSPA2 antibody included
  • IHC staining of human testis using 66291-1-Ig (same clone as 66291-1-PBS)

    IHC staining of human testis using 66291-1-Ig (same clone as 66291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66291-1-Ig (HSPA2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSPA2 antibody included
  • IHC staining of human testis using 66291-1-Ig (same clone as 66291-1-PBS)

    IHC staining of human testis using 66291-1-Ig (same clone as 66291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66291-1-Ig (HSPA2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSPA2 antibody included

HSPA2 Monoclonal antibody, PBS Only

HSPA2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66291-1-PBS

产品说明书

CloneNo. 1A4A9

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, mouse

应用

WB, IHC, Indirect ELISA

别名

Heat shock 70 kDa protein 2, heat shock 70kDa protein 2, HSP70 2, HSP70 3, HSPA2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HSPA2 Ready-To-Use IHC Kit. HSPA2 primary antibody included.

产品信息

66291-1-PBS targets HSPA2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat, mouse
免疫原 HSPA2 fusion protein Ag24228 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock 70kDa protein 2
别名 Heat shock 70 kDa protein 2, heat shock 70kDa protein 2, HSP70 2, HSP70 3, HSPA2
计算分子量 639 aa, 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC036107
基因名称 HSPA2
Gene ID (NCBI) 3306
RRIDAB_2881674
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP54652
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)