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验证数据展示

  • WB analysis using 67173-1-Ig

    WB analysis using 67173-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67173-1-Ig (HSPA4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67173-1-Ig

    WB analysis of HEK-293 using 67173-1-Ig

    WB result of HSPA4 antibody (67173-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPA4 transfected HEK-293 cells.

  • Featured Product
  • KD/KO验证

HSPA4 Monoclonal antibody

HSPA4 Monoclonal Antibody for WB, ELISA
Cat No. 67173-1-Ig

产品说明书

CloneNo. 2F2C12

宿主/亚型

Mouse / IgM

种属反应性

Human, pig

应用

WB, ELISA

别名

APG 2, APG2, Heat shock 70 kDa protein 4, heat shock 70kDa protein 4, HS24/P52, HSP70, hsp70RY, HSPA4, RY

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, pig brain tissue, HepG2 cells, L02 cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67173-1-Ig targets HSPA4 in WB, ELISA applications and shows reactivity with Human, pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, pig
免疫原 HSPA4 fusion protein Ag16624 种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock 70kDa protein 4
别名 APG 2, APG2, Heat shock 70 kDa protein 4, heat shock 70kDa protein 4, HS24/P52, HSP70, hsp70RY, HSPA4, RY
计算分子量 840 aa, 94 kDa
观测分子量 110 kDa
GenBank蛋白编号BC110861
基因名称 HSPA4
Gene ID (NCBI) 3308
RRIDAB_2882469
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP34932
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSPA4 (also known as Apg-2, HSP70RY) is a 110 kDa cytosolic protein, a member of the heat-shock protein 110 (Hsp 110) subfamily of Hsp70 proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA4 is ubiquitously expressed and its expression is not heat inducible. Overexpression of HSPA4 has been reported in some leukemia and solid tumors.

实验方案

Product Specific Protocols
WB protocol for HSPA4 antibody 67173-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols