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验证数据展示

  • WB analysis using 68059-1-Ig

    WB analysis using 68059-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68059-1-Ig (HSPB11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68059-1-Ig

    WB analysis of HeLa using 68059-1-Ig

    WB result of HSPB11 antibody (68059-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPB11 transfected HeLa cells.

  • Featured Product
  • KD/KO Validated

HSPB11 Monoclonal antibody

HSPB11 Monoclonal Antibody for WB, ELISA
Cat No. 68059-1-Ig

Print datasheet

CloneNo. 2D11B3

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, ELISA

Synonyms

Heat shock protein beta-11, Heat shock protein beta 11, FAP232, CFAP232, C1orf41

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inNCCIT cells, HeLa cells, LNCaP cells, HEK-293 cells, T-47D cells, Jurkat cells, K-562 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68059-1-Ig targets HSPB11 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen HSPB11 fusion protein Ag8804 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name heat shock protein family B (small), member 11
Synonyms Heat shock protein beta-11, Heat shock protein beta 11, FAP232, CFAP232, C1orf41
Calculated Molecular Weight 144 aa, 16 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC005245
Gene Symbol HSPB11
Gene ID (NCBI) 51668
RRIDAB_2918800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y547
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSPB11 (Heat shock protein beta-11), also known as IFT25, was originally identified as a small heat shock protein. Recently it has been identified as a component of IFT complex B. It has been demonstrated that IFT25 is not required for ciliary assembly but is required for proper Hedgehog signaling, which in mammals occurs within cilia. Cilia lacking IFT25 have defects in the signal-dependent transport of multiple Hedgehog components and fail to activate the pathway upon stimulation.

实验方案

Product Specific Protocols
WB protocol for HSPB11 antibody 68059-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols