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验证数据展示

  • WB analysis of PC-3 using 27771-1-AP

    WB analysis of PC-3 using 27771-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 27771-1-AP (HSPBAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 27771-1-AP

    IHC staining of mouse kidney using 27771-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27771-1-AP (HSPBAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HSPBAP1 Polyclonal antibody

HSPBAP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27771-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, IHC, ELISA

别名

HSPB1 associated protein 1, HSPBAP1, PASS1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells
Positive IHC detected inmouse kidney tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27771-1-AP targets HSPBAP1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 HSPBAP1 fusion protein Ag26808 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 HSPB (heat shock 27kDa) associated protein 1
别名 HSPB1 associated protein 1, HSPBAP1, PASS1
观测分子量 55 kDa
GenBank蛋白编号BC011897
基因名称 HSPBAP1
Gene ID (NCBI) 79663
RRIDAB_2880968
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96EW2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSPBAP1, also named as Protein associated with small stress protein 1, is a widely expressed 488 amino acid protein. HSPBAP1 may play a role in regulating stress response in the living cell(PMID: 11978969). A chromosomal aberration involving HSPBAP1 has been found in a family with renal carcinoma (PMID:12939738). HSPBAP1 has multiple isoforms with MW 55, 50, 32 and 25 kDa.

实验方案

Product Specific Protocols
WB protocol for HSPBAP1 antibody 27771-1-APDownload protocol
IHC protocol for HSPBAP1 antibody 27771-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols