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HSPBAP1 Polyclonal antibody

HSPBAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27771-1-AP

Print datasheet

Synonyms

HSPB1 associated protein 1, HSPBAP1, PASS1

验证数据展示

  • WB analysis of PC-3 using 27771-1-AP

    WB analysis of PC-3 using 27771-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 27771-1-AP (HSPBAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 27771-1-AP

    IHC staining of mouse kidney using 27771-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27771-1-AP (HSPBAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inPC-3 cells
Positive IHC detected inmouse kidney tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27771-1-AP targets HSPBAP1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen HSPBAP1 fusion protein Ag26808 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HSPB (heat shock 27kDa) associated protein 1
Synonyms HSPB1 associated protein 1, HSPBAP1, PASS1
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC011897
Gene Symbol HSPBAP1
Gene ID (NCBI) 79663
RRIDAB_2880968
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96EW2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSPBAP1, also named as Protein associated with small stress protein 1, is a widely expressed 488 amino acid protein. HSPBAP1 may play a role in regulating stress response in the living cell(PMID: 11978969). A chromosomal aberration involving HSPBAP1 has been found in a family with renal carcinoma (PMID:12939738). HSPBAP1 has multiple isoforms with MW 55, 50, 32 and 25 kDa.

实验方案

Product Specific Protocols
WB protocol for HSPBAP1 antibody 27771-1-APDownload protocol
IHC protocol for HSPBAP1 antibody 27771-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols