验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, A549 cells, human heart tissue, human skeletal muscle tissue, human testis tissue, Jurkat cells, MCF-7 cells, mouse brain tissue, mouse testis tissue, rat testis tissue |
Positive IHC detected in | human kidney tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10211-1-AP targets HSPBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | HSPBP1 fusion protein Ag0282 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1 |
Synonyms | Hsp70-binding protein 1, Hsp70 interacting protein 2, Hsp70 interacting protein 1, Hsp70 binding protein 2, Hsp70 binding protein 1 |
Calculated Molecular Weight | 39 kDa |
Observed Molecular Weight | 39 kDa |
GenBank Accession Number | BC002373 |
Gene Symbol | HSPBP1 |
Gene ID (NCBI) | 23640 |
RRID | AB_2121242 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NZL4 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hsp70-interacting protein (HSPBP1)is a protein of approximately 40 kilodaltons detected in cell extracts. Its mRNA is present in all tissues analyzed but is most abundant in heart and skeletal muscle. HSPBP1 binds the ATPase domain of Hsp70 and inhibits approximately 90% of the Hsp40-activated Hsp70 ATPase activity by preventing ATP binding to Hsp70.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for HSPBP1 antibody 10211-1-AP | Download protocol |
IHC protocol for HSPBP1 antibody 10211-1-AP | Download protocol |
IF protocol for HSPBP1 antibody 10211-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Res Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification. | ||
Mol Immunol HSPBP1 facilitates cellular RLR-mediated antiviral response by inhibiting the K48-linked ubiquitination of RIG-I.
|