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  • Featured Product
  • KD/KO Validated

HSPBP1 Polyclonal antibody

HSPBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10211-1-AP

Print datasheet

Synonyms

Hsp70-binding protein 1, Hsp70 interacting protein 2, Hsp70 interacting protein 1, Hsp70 binding protein 2, Hsp70 binding protein 1

验证数据展示

  • WB analysis using 10211-1-AP

    WB analysis using 10211-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10211-1-AP

    WB analysis using 10211-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 10211-1-AP

    IHC staining of human kidney using 10211-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10211-1-AP (HSPBP1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 10211-1-AP

    IHC staining of human kidney using 10211-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10211-1-AP (HSPBP1 antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 10211-1-AP

    IF Staining of HeLa using 10211-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSPBP1 antibody (10211-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, human heart tissue, human skeletal muscle tissue, human testis tissue, Jurkat cells, MCF-7 cells, mouse brain tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman kidney tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10211-1-AP targets HSPBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen HSPBP1 fusion protein Ag0282 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1
Synonyms Hsp70-binding protein 1, Hsp70 interacting protein 2, Hsp70 interacting protein 1, Hsp70 binding protein 2, Hsp70 binding protein 1
Calculated Molecular Weight 39 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberBC002373
Gene Symbol HSPBP1
Gene ID (NCBI) 23640
RRIDAB_2121242
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZL4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hsp70-interacting protein (HSPBP1)is a protein of approximately 40 kilodaltons detected in cell extracts. Its mRNA is present in all tissues analyzed but is most abundant in heart and skeletal muscle. HSPBP1 binds the ATPase domain of Hsp70 and inhibits approximately 90% of the Hsp40-activated Hsp70 ATPase activity by preventing ATP binding to Hsp70.

实验方案

Product Specific Protocols
WB protocol for HSPBP1 antibody 10211-1-APDownload protocol
IHC protocol for HSPBP1 antibody 10211-1-APDownload protocol
IF protocol for HSPBP1 antibody 10211-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cancer Res

Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.

Authors - Bingteng Xie
View Article
humanWB

Mol Immunol

HSPBP1 facilitates cellular RLR-mediated antiviral response by inhibiting the K48-linked ubiquitination of RIG-I.

Authors - Ya-Xian Yang
  • KD Validated
View Article