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HSPH1 Monoclonal antibody, PBS Only

HSPH1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3H5A5

Cat no : 66723-1-PBS

Print datasheet

Synonyms

Antigen NY CO 25, DKFZp686M05240, Heat shock 110 kDa protein, Heat shock protein 105 kDa, HSP105, HSP105A, HSP105B, HSP110, HSPH1, KIAA0201, NY CO 25

验证数据展示

  • WB analysis using 66723-1-Ig (same clone as 66723-1-PBS)

    WB analysis using 66723-1-Ig (same clone as 66723-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66723-1-Ig (HSPH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66723-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 66723-1-Ig (same clone as 66723-1-PBS)

    IHC staining of mouse testis using 66723-1-Ig (same clone as 66723-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66723-1-Ig (HSPH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66723-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66723-1-Ig (same clone as 66723-1-PBS)

    IF Staining of HeLa using 66723-1-Ig (same clone as 66723-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66723-1-Ig (HSPH1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66723-1-PBS in a different storage buffer formulation.

产品信息

66723-1-PBS targets HSPH1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse
Immunogen HSPH1 fusion protein Ag27521 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name heat shock 105kDa/110kDa protein 1
Synonyms Antigen NY CO 25, DKFZp686M05240, Heat shock 110 kDa protein, Heat shock protein 105 kDa, HSP105, HSP105A, HSP105B, HSP110, HSPH1, KIAA0201, NY CO 25
Calculated Molecular Weight 858 aa, 97 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC037553
Gene Symbol HSPH1
Gene ID (NCBI) 10808
RRIDAB_2882074
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ92598
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSP105, also known as HSP110 or HSPH1, belongs to the heat shock protein (HSP) family. Human HSP105 is a high-molecular-weight chaperone protein expressed at constitutively low levels as a cytoplasmic α-isoform and as an inducible nuclear β-isoform on exposure to various forms of stress. HSP105 is constitutively overexpressed in several solid tumors, including melanoma, breast, thyroid, and gastroenteric cancers, and exerts antiapoptotic functions. Recently HSP105 has been identified as a novel candidate biomarker of lymphoma aggressiveness. Western blot analysis using this antibody detected the phosphorylated protein around 110 kDa in various lysates.