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HTH-01-015

HTH-01-015 是一种选择性 NUAK1 抑制剂，IC50为 100 nM。它抑制 NUAK1 的效力比抑制 NUAK2 高 100 倍以上，IC50大于10 μM。

CAS号

1613724-42-7

分子式

C26H28N8O

主要靶点

AMPK

仅限科研使用

Cat No : CM02686

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Synonyms

验证数据展示

  • CAS No.: 1613724-42-7

    HTH-01-015
    CAS#: 1613724-42-7

产品信息

HTH-01-015 is a selective NUAK1 inhibitor (IC50=100 nM).

CAS号 1613724-42-7
分子式 C26H28N8O
主要靶点 AMPK
主要通路 表观遗传|PI3K/Akt/mTOR信号通路
分子量 468.55
纯度 98.10%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 Powder: -20°C for 3 years | In solvent: -80°C for 1 year
别名

靶点活性

NUAK1:100 nM

体外活性

In HEK-293 cells express NUAK1 as well as NUAK2, HTH-01-015 suppresses NUAK1-mediated MYPT1 phosphorylation. HTH-01-015 suppresses cell migration In NUAK1+/+ MEFs, and inhibit U2OS cell invasion. Moreover, HTH-01-015 inhibits cell proliferation in both cell lines. [1] HTH-01-015 inhibitors markedly restricted cells from entering into mitosis in U2OS cells. [2]

溶解度

DMSO:54 mg/mL (115.2 mM),H2O:<1 mg/mL,Ethanol:28 mg/mL (59.8 mM)

细胞实验

Cell proliferation assays are carried out colorimetrically in 96-well plates using the CellTiter 96? AQueous Non-Radioactive Cell Proliferation Assay kit following the manufacturer's protocol. Initially, 2000 cells per well are seeded for U2OS cells and 3000 cells per well are seeded for MEFs. The proliferation assays are carried out over 5 days in the presence or absence of 10 μM HTH-01-015.(Only for Reference)

参考文献

1.Banerjee S, et al. Biochem J. 2014, 457(1), 215-225.
2.Banerjee S, et al. Biochem J. 2014, 461(2), 233-245.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

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