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验证数据展示

  • WB analysis using 26438-1-AP

    WB analysis using 26438-1-AP

    H9C2 cells were subjected to SDS PAGE followed by western blot with 26438-1-AP (5HT2A Receptor antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 26438-1-AP

    IHC staining of human brain using 26438-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26438-1-AP (5HT2A Receptor Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human brain using 26438-1-AP

    IHC staining of human brain using 26438-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26438-1-AP (5HT2A Receptor Antibody) at dilution of 1:200 (under 10x lens).

5HT2A Receptor Polyclonal antibody

5HT2A Receptor Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26438-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, ELISA

别名

5 HT 2, 5 HT 2A, 5 HT2A, 5HT2A Receptor, HTR2, HTR2A, Serotonin receptor 2A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inH9C2 cells
Positive IHC detected inhuman brain tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26438-1-AP targets 5HT2A Receptor in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat
免疫原 5HT2A Receptor fusion protein Ag24056 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 5-hydroxytryptamine (serotonin) receptor 2A
别名 5 HT 2, 5 HT 2A, 5 HT2A, 5HT2A Receptor, HTR2, HTR2A, Serotonin receptor 2A
计算分子量 471 aa, 53 kDa
观测分子量53 kDa
GenBank蛋白编号BC074848
基因名称 5HT2A Receptor
Gene ID (NCBI) 3356
RRIDAB_3085872
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP28223
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The 5HT2A Receptor gene (HTR2A) codes for a G-protein coupled receptor and belongs to the G-protein coupled receptor 1 family. HTR2A plays a key role in serotonin pathway regulation. HTR2A affects neural activity, perception, cognition, and mood and functions as the main target of atypical antipsychotic drugs (PMID: 17691947, 21550210). 5HT2A Receptor has 2 isoforms with the molecular mass of 44 and 53 kDa.

实验方案

Product Specific Protocols
WB protocol for 5HT2A Receptor antibody 26438-1-APDownload protocol
IHC protocol for 5HT2A Receptor antibody 26438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols