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验证数据展示

  • WB analysis using 29685-1-AP

    WB analysis using 29685-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29685-1-AP (HTR3A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 29685-1-AP

    IHC staining of human small intestine using 29685-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 29685-1-AP (HTR3A antibody) at dilution of 1:200 (under 40x lens).

HTR3A Polyclonal antibody

HTR3A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29685-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, IHC, ELISA

别名

5 HT 3, 5 HT3 A, 5 HT3A, 5 HT3R, 5 hydroxytryptamine receptor 3, 5HT3R, HTR3, HTR3A, Serotonin receptor 3A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse lung tissue, mouse testis tissue
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29685-1-AP targets HTR3A in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 HTR3A fusion protein Ag31317 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 5-hydroxytryptamine (serotonin) receptor 3A
别名 5 HT 3, 5 HT3 A, 5 HT3A, 5 HT3R, 5 hydroxytryptamine receptor 3, 5HT3R, HTR3, HTR3A, Serotonin receptor 3A
计算分子量 55 kDa
观测分子量55 kDa
GenBank蛋白编号BC002354
基因名称 HTR3A
Gene ID (NCBI) 3359
RRIDAB_2918338
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP46098
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

HTR3A (5-hydroxytryptamine receptor 3A, 5-HT3A) belongs to the ligand-gated ion channel receptor superfamily. It is the subunit A of the type 3 receptor for 5-hydroxytryptamine (5-HT, serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. So far, five distinct 5-HT3 receptor subunits (A-E) have been identified. HTR3A can form functional homomers or heteromers with HTR3B or HTR3C or HTR3D or HTR3E. 5-HT3 receptors are capable of mediating fast excitatory neurotransmission in the CNS and peripheral nervous system (PNS). (PMID: 23038271; 17073663)

实验方案

Product Specific Protocols
WB protocol for HTR3A antibody 29685-1-APDownload protocol
IHC protocol for HTR3A antibody 29685-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols