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验证数据展示

  • WB analysis using 29685-1-AP

    WB analysis using 29685-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29685-1-AP (HTR3A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 29685-1-AP

    IHC staining of human small intestine using 29685-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 29685-1-AP (HTR3A antibody) at dilution of 1:200 (under 40x lens).

HTR3A Polyclonal antibody

HTR3A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29685-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Synonyms

5 HT 3, 5 HT3 A, 5 HT3A, 5 HT3R, 5 hydroxytryptamine receptor 3, 5HT3R, HTR3, HTR3A, Serotonin receptor 3A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated

Size: 
50 μL
100 μL
150 μL

¥2200/ 100 μL

浓度: 600 μg/ml

库存：现货

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经过测试的应用

Positive WB detected inmouse lung tissue, mouse testis tissue
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29685-1-AP targets HTR3A in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen HTR3A fusion protein Ag31317 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 5-hydroxytryptamine (serotonin) receptor 3A
Synonyms 5 HT 3, 5 HT3 A, 5 HT3A, 5 HT3R, 5 hydroxytryptamine receptor 3, 5HT3R, HTR3, HTR3A, Serotonin receptor 3A
Calculated Molecular Weight 55 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC002354
Gene Symbol HTR3A
Gene ID (NCBI) 3359
RRIDAB_2918338
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP46098
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

HTR3A (5-hydroxytryptamine receptor 3A, 5-HT3A) belongs to the ligand-gated ion channel receptor superfamily. It is the subunit A of the type 3 receptor for 5-hydroxytryptamine (5-HT, serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. So far, five distinct 5-HT3 receptor subunits (A-E) have been identified. HTR3A can form functional homomers or heteromers with HTR3B or HTR3C or HTR3D or HTR3E. 5-HT3 receptors are capable of mediating fast excitatory neurotransmission in the CNS and peripheral nervous system (PNS). (PMID: 23038271; 17073663)

实验方案

Product Specific Protocols
WB protocol for HTR3A antibody 29685-1-APDownload protocol
IHC protocol for HTR3A antibody 29685-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols