验证数据展示
经过测试的应用
| Positive IHC detected in | human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 2 publications below |
产品信息
21165-1-AP targets HTR4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 | HTR4 fusion protein Ag15226 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 5-hydroxytryptamine (serotonin) receptor 4 |
| 别名 | 5-HT-4, 5-HT4, 5 hydroxytryptamine receptor 4, 5 HT4R, 5 HT4 |
| 计算分子量 | 388 aa, 44 kDa |
| GenBank蛋白编号 | BC074755 |
| 基因名称 | HTR4 |
| Gene ID (NCBI) | 3360 |
| RRID | AB_11182374 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13639 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for HTR4 antibody 21165-1-AP | Download protocol |
| IF protocol for HTR4 antibody 21165-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother Enteromorpha and polysaccharides from enteromorpha ameliorate loperamide-induced constipation in mice. | ||
Nutrients Living, Heat-Killed Limosilactobacillus mucosae and Its Cell-Free Supernatant Differentially Regulate Colonic Serotonin Receptors and Immune Response in Experimental Colitis | ||
J Dairy Sci Synbiotic yogurt containing konjac mannan oligosaccharides and Bifidobacterium animalis ssp. lactis BB12 alleviates constipation in mice by modulating the stem cell factor (SCF)/c-Kit pathway and gut microbiota. | ||
Pharm Biol Prokinetic effects of Citrus reticulata and Citrus aurantium extract with/without Bupleurum chinense using multistress-induced delayed gastric emptying models | ||
J Nutr Limosilactobacillus mucosae and Lactobacillus amylovorus protect against experimental colitis via upregulation of colonic HTR4 and TGF-beta 2 |