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验证数据展示

  • WB analysis using 29618-1-AP

    WB analysis using 29618-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29618-1-AP (HTRA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 29618-1-AP

    IHC staining of human skin cancer using 29618-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 29618-1-AP (HTRA1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 29618-1-AP

    IF Staining of HepG2 using 29618-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HTRA1 antibody (29618-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

HTRA1 Polyclonal antibody

HTRA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29618-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PRSS11, HtrA, EC:3.4.21.-

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human placenta tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29618-1-AP targets HTRA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen HTRA1 fusion protein Ag31194 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HtrA serine peptidase 1
Synonyms PRSS11, HtrA, EC:3.4.21.-
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberNM_002775
Gene Symbol HTRA1
Gene ID (NCBI) 5654
RRIDAB_3086146
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92743
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HTRA1, also named as HTRA, PRSS11 and L56, belongs to the peptidase S1B family. It is a protease that regulates the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. HTRA1 represses signaling by TGF-beta family members. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). The 51 kDa HTRA1 was found at varying levels in the majority samples tested, whereas the 42 kDa form of HTRA1 was identified in degenerated intervertebral disc protein samples(PMID:22556410). The short truncated monomer 37 kDa and dimer 74 kDa are proved (PMID:25329061). The antibody is specific to HTRA1.

实验方案

Product Specific Protocols
WB protocol for HTRA1 antibody 29618-1-APDownload protocol
IHC protocol for HTRA1 antibody 29618-1-APDownload protocol
IF protocol for HTRA1 antibody 29618-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols