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  • Featured Product
  • KD/KO Validated

HTRA2 Polyclonal antibody, PBS Only

HTRA2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15775-1-PBS

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Synonyms

验证数据展示

  • WB analysis of HeLa using 15775-1-AP (same clone as 15775-1-PBS)

    WB analysis of HeLa using 15775-1-AP (same clone as 15775-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 15775-1-AP (same clone as 15775-1-PBS)

    IHC staining of human kidney using 15775-1-AP (same clone as 15775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 15775-1-AP (HTRA2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 15775-1-AP (same clone as 15775-1-PBS)

    IHC staining of human kidney using 15775-1-AP (same clone as 15775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 15775-1-AP (HTRA2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

  • IP experiment of Raji using 15775-1-AP (same clone as 15775-1-PBS)

    IP experiment of Raji using 15775-1-AP (same clone as 15775-1-PBS)

    IP result of anti-HTRA2 (IP:15775-1-AP, 4ug; Detection:15775-1-AP 1:500) with Raji cells lysate 2000ug. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

产品信息

15775-1-PBS targets HTRA2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, monkey
Immunogen HTRA2 fusion protein Ag8455 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HtrA serine peptidase 2
Synonyms
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC000096
Gene Symbol HTRA2
Gene ID (NCBI) 27429
RRIDAB_2122835
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43464
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HTRA2(High temperature requirement protein A2) is also named as OMI, PRSS25 and belongs to the peptidase S1B family. HTRA2 normally exists in the mitochondria, can cause MMP3 activation in the cytosol under a cell stress condition, which can ultimately lead to demise of dopaminergic neuronal cells(PMID: 22265821). It inhibits mitochondrial superoxide generation, stabilizes mitochondrial membrane potential, and prevents apoptosis at baseline and in response to extracellular inducers of mitochondrial stress(PMID: 18772386). This protein has 4 isoforms produced by alternative splicing.