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验证数据展示

  • IF Staining of HEK-293 using 19957-1-AP

    IF Staining of HEK-293 using 19957-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using Huntingtin antibody (19957-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of mouse brain using 19957-1-AP

    IHC staining of mouse brain using 19957-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19957-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HTT antibody included
  • IHC staining of mouse brain using 19957-1-AP

    IHC staining of mouse brain using 19957-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19957-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HTT antibody included

Huntingtin Polyclonal antibody

Huntingtin Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 19957-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

HTT, IT15, Huntington disease protein, Huntingtin, myristoylated N-terminal fragment, HD protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, SH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy HTT Ready-To-Use IHC Kit. HTT primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19957-1-AP targets Huntingtin in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 huntingtin
别名 HTT, IT15, Huntington disease protein, Huntingtin, myristoylated N-terminal fragment, HD protein
计算分子量 348 kDa
GenBank蛋白编号NM_002111
基因名称 Huntingtin
Gene ID (NCBI) 3064
RRIDAB_2878629
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42858
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HTT, also named as HD and IT15, belongs to the huntingtin family. HTT may play a role in microtubule-mediated transport or vesicle function. Defects in HTT are the cause of Huntington disease (HD) which is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. The antibody recognizes the C-term of HTT.

实验方案

Product Specific Protocols
IHC protocol for Huntingtin antibody 19957-1-APDownload protocol
IF protocol for Huntingtin antibody 19957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols