HVCN1 Polyclonal antibody
HVCN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Raji cells, PC-3 cells |
Positive IP detected in | PC-3 cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | PC-3 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
产品信息
14162-1-AP targets HVCN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | IHC |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | HVCN1 fusion protein Ag5350 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | hydrogen voltage-gated channel 1 |
Synonyms | VSOP, Voltage-gated hydrogen channel 1, Voltage sensor domain-only protein, UNQ578/PRO1140, Hydrogen voltage-gated channel 1 |
Calculated Molecular Weight | 273 aa, 32 kDa |
Observed Molecular Weight | 28-32 kDa, ~60 kDa |
GenBank Accession Number | BC032672 |
Gene Symbol | HVCN1 |
Gene ID (NCBI) | 84329 |
RRID | AB_2878023 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q96D96 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for HVCN1 antibody 14162-1-AP | Download protocol |
IHC protocol for HVCN1 antibody 14162-1-AP | Download protocol |
IF protocol for HVCN1 antibody 14162-1-AP | Download protocol |
IP protocol for HVCN1 antibody 14162-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Immunol Diagnostic and Predictive Value of Immune-Related Genes in Crohn's Disease. |