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Haptoglobin Monoclonal antibody, PBS Only

Haptoglobin Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B4B9

Cat no : 66229-1-PBS

Print datasheet

Synonyms

BP, HAPT, haptoglobin, HP, HP2 ALPHA 2, HPA1S, Zonulin

验证数据展示

  • WB analysis of human plasma using 66229-1-Ig (same clone as 66229-1-PBS)

    WB analysis of human plasma using 66229-1-Ig (same clone as 66229-1-PBS)

    0.5 μl human plasma was subjected to SDS PAGE followed by western blot with 66229-1-Ig (HP Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66229-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66229-1-Ig (same clone as 66229-1-PBS)

    IHC staining of human liver cancer using 66229-1-Ig (same clone as 66229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66229-1-Ig (Haptoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66229-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HP antibody included
  • IHC staining of human liver cancer using 66229-1-Ig (same clone as 66229-1-PBS)

    IHC staining of human liver cancer using 66229-1-Ig (same clone as 66229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66229-1-Ig (Haptoglobin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66229-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HP antibody included
Planning an IHC experiment? We recommend our IHCeasy HP Ready-To-Use IHC Kit. HP primary antibody included.

产品信息

66229-1-PBS targets Haptoglobin in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, pig
Immunogen Haptoglobin fusion protein Ag9927 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name haptoglobin
Synonyms BP, HAPT, haptoglobin, HP, HP2 ALPHA 2, HPA1S, Zonulin
Calculated Molecular Weight281aa,31 kDa; 228aa,25 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC058031
Gene Symbol HP
Gene ID (NCBI) 3240
RRIDAB_2881620
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00738
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HP(Haptoglobin) is also named as zonulin and belongs to the peptidase S1 family. HP, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha(16 kDa and 9 kDa) and 2 beta(40 kDa) polypeptides that are covalently associated by disulfide bonds. In most species, apart from ruminants, Hp has a molecular mass of 100 kDa, consisting of two subunits of 40 kDa and two subunits of 9 kDa, although in a few species, such as man, genetic variant of Hp forms polymers of higher mass(PMID:2361363). Recent studies of haptoglobin show that certain oligosaccharide structures predominate in different diseases. For example, a highly-fucosylated structure is found in breast cancer and ovarian cancer, highly-sialylated structures in Crohn’s disease and highly branched structures in alcoholic liver disease and fucosylated haptoglobin is a good serum marker for pancreatic cancer.(PMID:16385567).