验证数据展示
经过测试的应用
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83993-4-RR targets Histone H1.0 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IHC, IF/ICC, ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | Histone H1.0 fusion protein Ag9982 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | H1 histone family, member 0 |
| Synonyms | H1F0, H1FV, H1-0, H10, H1 histone family, member 0 |
| Calculated Molecular Weight | 21 kDa |
| GenBank Accession Number | BC000145 |
| Gene Symbol | Histone H1.0 |
| Gene ID (NCBI) | 3005 |
| RRID | AB_3671569 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P07305 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in forming higher order structure in chromatin and maintaining overall chromatin compaction. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.Histone H1.0 (H1F0,H1FV) is a linker histone that is widely expressed in many tissues and almost all vertebrates, unlike some other linker histones.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Histone H1.0 antibody 83993-4-RR | Download protocol |
| IF protocol for Histone H1.0 antibody 83993-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
