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验证数据展示

  • IF Staining of HepG2 using 83993-4-RR

    IF Staining of HepG2 using 83993-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Histone H1.0 antibody (83993-4-RR, Clone: 241147F6 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of mouse colon using 83993-4-RR

    IHC staining of mouse colon using 83993-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83993-4-RR (Histone H1.0 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Histone H1.0 antibody included

Histone H1.0 Recombinant antibody

Histone H1.0 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 83993-4-RR

产品说明书

CloneNo. 241147F6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

H1F0, H1FV, H1-0, H10, H1 histone family, member 0

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy Histone H1.0 Ready-To-Use IHC Kit. Histone H1.0 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83993-4-RR targets Histone H1.0 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Histone H1.0 fusion protein Ag9982 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 H1 histone family, member 0
别名 H1F0, H1FV, H1-0, H10, H1 histone family, member 0
计算分子量 21 kDa
GenBank蛋白编号BC000145
基因名称 Histone H1.0
Gene ID (NCBI) 3005
RRIDAB_3671569
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP07305
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in forming higher order structure in chromatin and maintaining overall chromatin compaction. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.Histone H1.0 (H1F0,H1FV) is a linker histone that is widely expressed in many tissues and almost all vertebrates, unlike some other linker histones.

实验方案

Product Specific Protocols
IHC protocol for Histone H1.0 antibody 83993-4-RRDownload protocol
IF protocol for Histone H1.0 antibody 83993-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols