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Histone H2A.XS139ph antibody (pAb) (H2AX)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Wide Range Predicted

Applications

IF, WB, Dot Blot, ICC

Cat No : 39117,39118 39117

Synonyms

histone H2A.X, histone H2AX, histone, H2A.X, H2AX, histone-H2AX, histone-H2A.X, histoneH2AX, histoneH2A.X, gammaH2AX, gammah2A.X, gamma-h2ax, gamma-h2a.x, gamma h2ax, gamma h2A.X, pAb, polyclonal, H2AX P-S139, H2A histone family member X, Histone H2 Phospho Ser139, Histone H2 Phosphorylated Ser139, Histone H2 Phosphorylated Serine 139, Histone H2 P-Ser139, Histone H2 Phospho S139, H2 Phospho Ser139, H2 P-Ser139, antibody, antibodies, H2AXS139Ph, hisvar, sample

验证数据展示

  • Histone H2AX phospho Ser139 antibody tested by immunofluorescence. HeLa cells stained with Histone H2AX phospho Ser139 antibody (1:500 dilution) using MAX Stain Immunofluorescence Tools. The HeLa cells were blocked with MAXblock Blocking Medium and stained with Histone H2AX phospho Ser139 antibody. Panel A: Untreated HeLa cells. Panel B: Cells fixed and stained 90 minutes after 3 Gy ionizing radiation treatment. Top images: Cells were stained with DAPI. Middle images: Same cells stained with Histone H2AX phospho Ser139 antibody. Bottom images: Merge of both images above. Images were made using Zeiss Axiovision with equivalent acquisition settings for direct comparison. Note Panel B-middle image, which shows intense nuclear clustering of ionizing radiation-induced phosphorylation of Ser139 of H2AX. In contrast, Panel A-middle image shows no detectable phosphorylated H2AX.

    Histone H2AX phospho Ser139 antibody tested by immunofluorescence. HeLa cells stained with Histone H2AX phospho Ser139 antibody (1:500 dilution) using MAX Stain Immunofluorescence Tools. The HeLa cells were blocked with MAXblock Blocking Medium and stained with Histone H2AX phospho Ser139 antibody. Panel A: Untreated HeLa cells. Panel B: Cells fixed and stained 90 minutes after 3 Gy ionizing radiation treatment. Top images: Cells were stained with DAPI. Middle images: Same cells stained with Histone H2AX phospho Ser139 antibody. Bottom images: Merge of both images above. Images were made using Zeiss Axiovision with equivalent acquisition settings for direct comparison. Note Panel B-middle image, which shows intense nuclear clustering of ionizing radiation-induced phosphorylation of Ser139 of H2AX. In contrast, Panel A-middle image shows no detectable phosphorylated H2AX.

  • Histone H2AX phospho Ser139 antibody tested by immunofluorescence. Etoposide-treated HeLa cells stained with Cat. No. 39117 (1:500 dilution, red) and counterstained with DAPI (blue).

    Histone H2AX phospho Ser139 antibody tested by immunofluorescence. Etoposide-treated HeLa cells stained with Cat. No. 39117 (1:500 dilution, red) and counterstained with DAPI (blue).

  • Histone H2AX phospho Ser139 antibody tested by Western blot. Western blot: Nuclear extract of U2OS cells (20 ug per lane) probed with Histone H2AX phospho Ser139 polyclonal antibody (1:500 dilution). Lane 1: untreated cells Lane 2: cells treated with camptothecin

    Histone H2AX phospho Ser139 antibody tested by Western blot. Western blot: Nuclear extract of U2OS cells (20 ug per lane) probed with Histone H2AX phospho Ser139 polyclonal antibody (1:500 dilution). Lane 1: untreated cells Lane 2: cells treated with camptothecin

产品信息

Tested Applications IF, WB, Dot Blot, ICC

Applications Validated by Active Motif: ICC/IF: 1:500 dilution WB*: 1:1,000 - 1:5,000 dilution DB: 0.1 ug/ml *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. Visit activemotif.com to download the protocol.
Tested Reactivity Human, Wide Range Predicted
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
ModificationPhosphorylated
Immunogen This Histone H2AX phospho Ser139 antibody was raised against a peptide including phosphoserine 139 of histone H2AX.
Full Name Histone H2A.XS139ph antibody (pAb) (H2AX)
Synonyms histone H2A.X, histone H2AX, histone, H2A.X, H2AX, histone-H2AX, histone-H2A.X, histoneH2AX, histoneH2A.X, gammaH2AX, gammah2A.X, gamma-h2ax, gamma-h2a.x, gamma h2ax, gamma h2A.X, pAb, polyclonal, H2AX P-S139, H2A histone family member X, Histone H2 Phospho Ser139, Histone H2 Phosphorylated Ser139, Histone H2 Phosphorylated Serine 139, Histone H2 P-Ser139, Histone H2 Phospho S139, H2 Phospho Ser139, H2 P-Ser139, antibody, antibodies, H2AXS139Ph, hisvar, sample
Molecular weight 15 kDa
GenBank accession numberNP_002096
RRIDAB_2793161
Purification Method Protein A Chromatography
Buffer PBS containing 30% glycerol, 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

Histone H2AX phospho Ser139 (H2AX, H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation.