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验证数据展示

  • WB analysis using 31048-1-AP

    WB analysis using 31048-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31048-1-AP (Histone H2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Histone H2B Polyclonal antibody

Histone H2B Polyclonal Antibody for WB, ELISA
Cat No. 31048-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

bA317E16.3, H2BFU, HIST1H2BA, histone cluster 1, H2ba, Histone H2B type 1 A, Histone H2B, testis, STBP, Testis specific histone H2B, TSH2B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31048-1-AP targets Histone H2B in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen Histone H2B fusion protein Ag33975 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histone cluster 1, H2ba
Synonyms bA317E16.3, H2BFU, HIST1H2BA, histone cluster 1, H2ba, Histone H2B type 1 A, Histone H2B, testis, STBP, Testis specific histone H2B, TSH2B
Observed Molecular Weight15 kDa
GenBank Accession NumberBC066242
Gene Symbol Histone H2B
Gene ID (NCBI) 255626
RRIDAB_3669832
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96A08
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones are the basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.Histone H2BC1 is testis specific.

实验方案

Product Specific Protocols
WB protocol for Histone H2B antibody 31048-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols