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Histone H3K18ac antibody (pAb)

Host / Isotype

Rabbit / Serum

Reactivity

Human, Wide Range Predicted

Applications

IF, WB, ChIP, Dot Blot, ICC

Cat No : 39129,39130 39129

Synonyms

histone H3, histone, H3, histone-H3, histoneH3, pAb, polyclonal, H3 AcK18, Histone H3 Acetyl Lys18, Histone H3 Acetyl K18, Histone H3 Ac Lys18, H3 Acetyl Lys18, H3 Acetyl K18, H3 Ac Lys18, Histone H3 Acetylated Lysine 18, Histone H3 Acetyl Lysine18, H3 Acetylated Lysine 18, H3 Acetyl Lysine18, antibody, antibodies, H3K18Ac, sample

验证数据展示

  • Histone H3 acetyl Lys18 pAb tested by immunofluorescence. Staining of HeLa cells with Histone H3 acetyl Lys18 pAb (1:500 dilution, top panel) and DAPI (bottom panel).

    Histone H3 acetyl Lys18 pAb tested by immunofluorescence. Staining of HeLa cells with Histone H3 acetyl Lys18 pAb (1:500 dilution, top panel) and DAPI (bottom panel).

  • Histone H3 acetyl Lys18 pAb tested by Western blot. HeLa acid extract probed with Histone H3 acetyl Lys18 polyclonal antibody (1:5,000 dilution). Lane 1: No treatment. Lane 2: Cells treated with sodium butyrate.

    Histone H3 acetyl Lys18 pAb tested by Western blot. HeLa acid extract probed with Histone H3 acetyl Lys18 polyclonal antibody (1:5,000 dilution). Lane 1: No treatment. Lane 2: Cells treated with sodium butyrate.

  • Histone H3 acetyl Lys18 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys18 pAb for acetyl Lys18 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Acetyl-Lys9 peptide. Lane 2: Unmodified Lys9 peptide. Lane 3: Acetyl-Lys14 peptide. Lane 4: Unmodified Lys14 peptide. Lane 5: Acetyl-Lys18 peptide. Lane 6: Unmodified Lys18 peptide. Lane 7: Acetyl-Lys23 peptide. Lane 8: Unmodified Lys23 peptide. Lane 9: Acetyl-Lys27 peptide. Lane 10: Unmodified Lys27 peptide.

    Histone H3 acetyl Lys18 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys18 pAb for acetyl Lys18 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Acetyl-Lys9 peptide. Lane 2: Unmodified Lys9 peptide. Lane 3: Acetyl-Lys14 peptide. Lane 4: Unmodified Lys14 peptide. Lane 5: Acetyl-Lys18 peptide. Lane 6: Unmodified Lys18 peptide. Lane 7: Acetyl-Lys23 peptide. Lane 8: Unmodified Lys23 peptide. Lane 9: Acetyl-Lys27 peptide. Lane 10: Unmodified Lys27 peptide.

产品信息

Tested Applications IF, WB, ChIP, Dot Blot, ICC

Applications Validated by Active Motif: ICC/IF: 1:500 - 1:1,000 dilution WB*: 1:1,000 - 1:5,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Human, Wide Range Predicted
Host / Isotype Rabbit / Serum
Class Polyclonal
Type Antibody
ModificationAcetylated
Immunogen This Histone H3 acetyl Lys18 antibody was raised against a peptide including acetyl-lysine 18 of histone H3.
Full Name Histone H3K18ac antibody (pAb)
Synonyms histone H3, histone, H3, histone-H3, histoneH3, pAb, polyclonal, H3 AcK18, Histone H3 Acetyl Lys18, Histone H3 Acetyl K18, Histone H3 Ac Lys18, H3 Acetyl Lys18, H3 Acetyl K18, H3 Ac Lys18, Histone H3 Acetylated Lysine 18, Histone H3 Acetyl Lysine18, H3 Acetylated Lysine 18, H3 Acetyl Lysine18, antibody, antibodies, H3K18Ac, sample
Molecular weight 17 kDa
GenBank accession numberNP_003522
RRIDAB_2793164
Purification Method None
Buffer Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. In estrogen-responsive genes, Histone H3 Lys18 is acetylated by CBP/p300 following estrogen stimulation, leading to acetylation of histone H3 Lys23 and methylation of Arg17 by CARM1. These events lead to transcriptional activation of the genes.