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  • Featured Product
  • KD/KO Validated

Hsc70 Monoclonal antibody, PBS Only

Hsc70 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A4B3

Cat no : 66442-1-PBS

Print datasheet

Synonyms

Heat shock 70 kDa protein 8, Heat shock cognate 71 kDa protein, Hsc70, HSC71, HSP71, HSP73, HSP7C, HSPA10, HSPA8

验证数据展示

  • WB analysis using 66442-1-Ig (same clone as 66442-1-PBS)

    WB analysis using 66442-1-Ig (same clone as 66442-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66442-1-Ig (Hsc70 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66442-1-Ig (same clone as 66442-1-PBS)

    WB analysis of HeLa using 66442-1-Ig (same clone as 66442-1-PBS)

    Western blot analysis of Hsc70 in various cell lines using 66442-1-Ig (Hsc70 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 66442-1-Ig (same clone as 66442-1-PBS)

    IF Staining of HEK-293 using 66442-1-Ig (same clone as 66442-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Hsc70 antibody (66442-1-Ig, Clone: 1A4B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

  • WB analysis of Recombinant protein using 66442-1-Ig (same clone as 66442-1-PBS)

    WB analysis of Recombinant protein using 66442-1-Ig (same clone as 66442-1-PBS)

    6*His tagged-recombinant HSP70 (291-641 aa) and HSC70 (346-646 aa) proteins were subjected to SDS PAGE followed by western blot with 66183-1-Ig (HSP70 antibody), 66442-1-Ig (HSC70 antibody), and 66005-1-Ig (His antibody), respectively, at dilution of 1:50000, incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66442-1-Ig (same clone as 66442-1-PBS)

    WB analysis of HEK-293 using 66442-1-Ig (same clone as 66442-1-PBS)

    WB result of Hsc70 antibody (66442-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Hsc70 transfected HEK-293 cells. This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66442-1-Ig (same clone as 66442-1-PBS)

    IHC staining of human ovary tumor using 66442-1-Ig (same clone as 66442-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66442-1-Ig (Hsc70 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSC70/HSPA8 antibody included
  • IHC staining of human ovary tumor using 66442-1-Ig (same clone as 66442-1-PBS)

    IHC staining of human ovary tumor using 66442-1-Ig (same clone as 66442-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66442-1-Ig (Hsc70 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSC70/HSPA8 antibody included
Planning an IHC experiment? We recommend our IHCeasy HSC70/HSPA8 Ready-To-Use IHC Kit. HSC70/HSPA8 primary antibody included.

产品信息

66442-1-PBS targets Hsc70 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Hsc70 fusion protein Ag17910 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name heat shock 70kDa protein 8
Synonyms Heat shock 70 kDa protein 8, Heat shock cognate 71 kDa protein, Hsc70, HSC71, HSP71, HSP73, HSP7C, HSPA10, HSPA8
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC007276
Gene Symbol HSC70
Gene ID (NCBI) 3312
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11142
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson's disease, indicating its implication in neurodegenerative disease. (PMID: 21832061)