验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HCT 116 cells, NCI-H1299 cells, K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy HuR/ELAVL1 Ready-To-Use IHC Kit. HuR/ELAVL1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82690-2-RR targets HuR/ELAVL1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | HuR/ELAVL1 fusion protein Ag2249 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R) |
| 别名 | ELAVL1, Hu antigen R, ELAV-like protein 1, ELAVL 1, ELAV1 |
| 计算分子量 | 326 aa, 36 kDa |
| 观测分子量 | 30-37 kDa |
| GenBank蛋白编号 | BC003376 |
| 基因名称 | HuR/ELAVL1 |
| Gene ID (NCBI) | 1994 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q15717 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HuR/ELAVL1 antibody 82690-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |