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Recombinant Human ACE2 protein (His Tag)

ED50

44-176 ng/mL

Species

Human

Purity

>95 %, SDS-PAGE

GeneID

59272

Accession

Q9BYF1

Cat No : Eg0074

Print datasheet

Synonyms

ACE2, ACE-2, ACEH

验证数据展示

  • Purity of Recombinant Human ACE2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human ACE2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human ACE2 (His tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD (hFc tag, Myc tag, His tag) with a linear range of 44-176 ng/mL.

    Immobilized Human ACE2 (His tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD (hFc tag, Myc tag, His tag) with a linear range of 44-176 ng/mL.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Human ACE2 (His tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD (hFc tag, Myc tag, His tag) with a linear range of 44-176 ng/mL.
Source HEK293-derived Human ACE2 protein Gln18-Ser740 (Accession# Q9BYF1) with a His tag at the N-terminus.
Predicted Molecular Mass 84.4 kDa
SDS-PAGE 85-105 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Angiotensin-converting enzyme 2 (ACE2), a critical regulator of the renin-angiotensin system (RAS), belongs to the peptidase M2 family. It plays an important role in cardiovascular homeostasis by regulating vascular tone, fluid, and electrolyte balance. ACE2 functions as a carboxymonopeptidase hydrolyzing the cleavage of a single C-terminal residue from Angiotensin-II (Ang-II), the key peptide hormone of RAS, to form Angiotensin-(1-7) (Ang-(1-7)), which binds to the G-protein-coupled Mas receptor and activates signaling pathways that counteract the pathways activated by Ang-II. ACE2 is expressed in a variety of tissues, including the kidneys, testes, heart, and intestines, and is particularly enriched in lung epithelium. Recent studies have shown that increased ACE2 expression is likely to help prevent secondary fibrosis changes following COVID-19 pneumonia.

References:

1. Li Q. et al. (2020). Clin Sci (Lond). 134:2581-2595.
2. Li Y. et al. (2020). Biochem Biophys Res Commun. 526:947-952.
3. Hikmet F. et al. (2020). Mol Syst Biol. 16:e9610.
4. Chaudhry F. et al. (2020). Open Heart. 7:e001424.