• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Recombinant Human CD155/PVR protein (hFc Tag)

ED50

25-100 ng/mL

Species

Human

Purity

>95 %, SDS-PAGE

GeneID

5817

Accession

NP_006496.4

Cat No : Eg0970

Print datasheet

Synonyms

CD155, FLJ25946, HVED, NECL5, PVR, PVS, TAGE4

验证数据展示

  • Purity of Recombinant Human CD155 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD155 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human TIGIT (His tag) at 2 μg/mL (100 μL/well) can bind Human CD155 (hFc tag) with a linear range of 25-100 ng/mL.

    Immobilized Human TIGIT (His tag) at 2 μg/mL (100 μL/well) can bind Human CD155 (hFc tag) with a linear range of 25-100 ng/mL.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Human TIGIT (His tag) at 2 μg/mL (100 μL/well) can bind Human CD155 (hFc tag) with a linear range of 25-100 ng/mL.
Source HEK293-derived Human CD155 protein Trp21-Asn343 (Accession# NP_006496.4) with a human IgG1 Fc tag at the C-terminus.
Predicted Molecular Mass 61.1 kDa
SDS-PAGE 70-95 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

PVR also known as CD155, is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. It contains three extracellular immunoglobulin-like domains, D1-D3, of which D1 is recognized by the virus. Mature human PVR consists of a 323 amino acid extracellular domain with one N-terminal V-type and two C2-type Ig-like domains, a 24 amino acid transmembrane segment, and a 50 amino acid cytoplasmic tail. PVR is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. PVR binds to two receptors of NK cells, CD96 and CD226, and accumulates at cell-cell contact sites, leading to the formation of mature immune synapses between NK cells and target cells. PVR serves as the entry receptor for poliovirus and thereby mediates human susceptibility to poliovirus infection.

References:

1. Mendelsohn CL. et al. (1989). Cell. 56(5):855-865.
2. Zhang P. et al. (2008). Proc Natl Acad Sci U S A. 105(47):18284-9.
3 .Pende D. et al. (2005). Mol Immunol. 42(4):463-469.
4. Fuchs A. et al. (2004). J Immunol. 172(7):3994-8.